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007			cr nn 008mamaa
008			100301s1999 xxu| o |||| 0|eng d
020			_a9781592594870
024	7		_a10.1385/0896035662 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR100 _b1999 EB
245	1	0	_aEnvironmental Monitoring of Bacteria _cedited by Clive Edwards
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (X, 333 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Biotechnology _x1940-607X _v12
505	0		_aSome Problems Posed by Natural Environments for Monitoring Microorganisms -- Sampling Sediment and Soil -- Sampling Water Bodies -- Dielectrophoresis -- Flow Cytometry and Cell Sorting -- Magnetic Particle-Based Separation Techniques for Monitoring Bacteria from Natural Environments -- DNA Extraction from Natural Environments -- Automated Sequencing of DNA Retrieved from Environmental Samples -- Analysis of DNA Sequences -- Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes Amplified Directly from Bacterial Communities in Soils and Sediments -- Recovery and Analysis of Ribosomal RNA Sequences from the Environment -- Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology -- Reporter Gene Expression for Monitoring Microorganisms in the Environment -- Characterizing Microorganisms in the Environment by Fatty Acid Analysis -- Fluorescent In Situ Hybridization and the Analysis of the Single Cell -- Specific Detection, Viability Assessment, and Macromolecular Staining of Bacteria for Flow Cytometry -- Confocal Laser Scanning Microscopy of Environmental Samples -- Monitoring Microbial Activities Using Membrane Inlet Mass Spectrometry -- Experimental Biofilms and Their Applications in the Study of Environmental Processes -- Establishment of Experimental Biofilms Using the Modified Robbins Device and Flow Cells.
520			_aIn Environmental Monitoring of Bacteria, Clive Edwards and his distinguished panel of researchers bring together a collection of detailed molecular methods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations. Many of the methods enable bacteria to be monitored in their natural environments without culturing, and specifically address the difficult problem that only a small proportion of bacteria in any ecosystem can be cultured by traditional methods. In addition to providing many eminently reproducible methods, the distinguished contributors review the problems of monitoring bacteria in natural environments and discuss the principles by which the methods work. Taking full advantage of the exciting research in recent years that has challenged some long-held principles of traditional microbiology, Environmental Monitoring of Bacteria gives timely and practical access to powerfultechniques that allow the study of bacterial ecology as communities, as single cells, or at the molecular level.
988			_aSpringer_Protocols_1999
650		7	_2embne _9150106 _aEcología microbiana
650		7	_2embne _9138901 _aBacterias _xEcología
776	0	8	_iPrinted edition: _z9781617370779
776	0	8	_iPrinted edition: _z9781489943231
776	0	8	_iPrinted edition: _z9780896035669
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896035662 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI