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008			100301s1995 xxu| o |||| 0|eng d
020			_a9781592595211
024	7		_a10.1385/089603285X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR187 .I45 _b1995 EB
245	0	0	_aImmunocytochemical Methods and Protocols _cedited by Lorette C. Javois
250			_a1st edition 1995
264		1	_aTotowa, NJ _bHumana Press _c1995
300			_a1 recurso en línea (XII, 435 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v34
505	0		_aOverview of Antibody Use in Immunocytochemistry -- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration -- Purification of Antibodies Using Ion-Exchange Chromatography -- Purification of Antibodies Using Affinity Chromatography -- Purification of Antibodies Using Protein A-Sepharose and FPLC -- Conjugation of Fluorochromes to Antibodies -- Biotinylation of Antibodies -- Overview of Cell Fixation and Permeabilization -- Preparation of Frozen Sections for Analysis -- Processing of Cell-Touch Preparations -- Processing of Cytological Specimens -- Processing of Tissue-Culture Cells -- Processing of Tissue Specimens -- Overview of Fluorophores -- Direct Immunofluorescent Labeling of Cells -- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts -- Overview of Antigen Detection Through Enzymatic Activity -- The Peroxidase-Antiperoxidase (PAP) Method -- The Avidin-Biotin Complex(ABC) Method -- Labeled Avidin Binding (LAB) Method* -- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue -- ELISA on Attachment-Dependent or Suspension Grown Cells -- Use of Immunogold with Silver Enhancement -- Overview of Flow Cytometry and Fluorescent Probes for Cytometry -- Tissue Disaggregation -- Indirect Immunofluorescent Labeling of Viable Cells -- Indirect Immunofluorescent Labeling of Fixed Cells -- Fluorescent Labeling of DNA -- Deparaffinization and Processing of Pathologic Material -- Assay for Phagocytosis -- Assay for Filamentous Actin -- Assay for Chemoattractant Binding -- Assay for Oxidative Metabolism -- Fixation and Embedding -- Preparation of Colloidal Gold -- Conjugation of Colloidal Gold to Proteins -- Colloidal Gold/Streptavidin Methods -- Pre-Embedding Labeling Methods -- Postembedding Labeling Methods -- Overview of Fluorescence Photomicrography -- Overview of Fluorescence Analysis with the Confocal Microscope -- Overview of Laser Microbeam Applications as Related to Antibody Targeting -- Nucleic Acid Immunocytochemistry -- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes -- Overview of Automated Immunostainers -- Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors.
520			_aThe principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years. During this time the use of immunocytochemical meth­ ods has spread to a wide array of biological disciplines. Early applica­ tions focused on the detection of microbial antigens in tissues, while more recent applications have used monoclonal antibodies to study cell differentiation during embryonic development. For a select few disci­ plines, volumes have been published focusing on the specific applica­ tion of immunocytochemical techniques to that discipline. What distinguishes the present book, Immunocytochemical Meth­ ods and Protocols, from earlier books is its broad appeal to researchers in all disciplines, including those in both research and clinical settings. The methods and protocols presented here are designed to be general in their application and the accompanying "Notes" provide invaluable assistance in adapting or troubleshooting the protocols. Interspersed throughout the book are chapters providing overviews of select topics related to immunocytochemistry.
988			_aSpringer_Protocols_1995
650		7	_2embne _9675983 _aInmunocitoquímica
776	0	8	_iPrinted edition: _z9780896032859
776	0	8	_iPrinted edition: _z9781489940063
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/089603285X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b01/2024 _dz _eIG _zSI