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008			130809s2013 xxu| o |||| 0|eng d
020			_a9781627035569
024	7		_a10.1007/978-1-62703-556-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP86 _b2013 EB
245	0	0	_aBiological Aging : _bMethods and Protocols _cedited by Trygve O. Tollefsbol
250			_a2nd edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 354 páginas) _b54 ilustraciones, 35 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1048
505	0		_aCell Senescence Culturing Methods -- Digital Image Analysis of Cells Stained with the Senescence-Associated -Galactosidase Assay -- Analysis of Biomarkers of Caloric Restriction in Aging Cells -- Cell Sorting of Young and Senescent Cells -- Studying the Replicative Life Span of Yeast Cells -- Methods for Creating Mutations in C. elegans that Extend Lifespan -- Aging Studies in Drosophila Melanogaster -- The Use of Calorie Restriction Mimetics to Study Aging -- Using Somatic-Cell Nuclear Transfer to Study Aging -- Induction of Cellular Senescence by Oncogenic RAS -- Methods of Cellular Senescence Induction Using Oxidative Stress -- Methods of Testing Pharmacological Drugs Effects on Aging and Life-span in Mice -- Mitochondria-targeted Anti-aging Gene Therapy with Adeno-associated Viral Vectors -- Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration -- Exometabolomic Mapping of Caenorhabditis elegans - A Tool to Non-Invasively Investigate Aging -- Detecting Polymorphisms in Human Longevity Studies: HLA typing and SNP Genotyping by Amplicon Sequencing -- Gel Electrophoresis-based Proteomics of Senescent Tissues -- Single-Cell Semiconductor Sequencing -- Application of DNA Microarray Technology to Gerontological Studies -- Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells -- Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging.
520			_aThe second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Biological Aging:  Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.
988			_aSpringer_Protocols_2013
650		7	_2embne _9144609 _aLongevidad
650		7	_2embne _9141231 _aEnvejecimiento _xAspectos fisiológicos
776	0	8	_iPrinted edition: _z9781627035576
776	0	8	_iPrinted edition: _z9781627035552
776	0	8	_iPrinted edition: _z9781493963133
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-556-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI