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008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592591176
024	7		_a10.1385/1592591175 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR406.2 .S56 _b2001 EB
245	0	0	_aSV40 Protocols _cedited by Leda Raptis
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XI, 310 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v165
505	0		_aPropagation of Wild-Type and Mutant SV40 -- Propagation and Assay of the JC Virus -- Propagation and Assay of BK Virus -- Molecular Methods for Identification and Genotyping of BK Virus -- Methods for Studying Interactions Between Simian Virus 40 T-Antigen and the Viral Origin of Replication -- Cell-Free Transcription of SV40 -- Quantitation and Structural Analysis of SV40 RNAs -- Generation of Recombinant SV40 Vectors for Gene Transfer -- Production of SV40 Proteins in Insect Cells and In Vitro Packaging of Virions and Pseudovirions -- Using Retroviral Vectors to Express SV40 Tumor Antigens -- Neoplastic Transformation Assays -- Methods to Assess Inhibition of Adipocyte Differentiation by SV40 Large T Antigen -- SV40-Mediated Immortalization -- Biology of p53 and SV40 Large T Association -- Binding of SV40 Large T Antigen to the Retinoblastoma Susceptibility Gene Product and Related Proteins -- In situ Electroporation for the Measurement of c-Ras Activation by SVLT -- Role of SV40 Small t in Cell Lysis, Transformation, and Signaling -- Cytotoxic T Lymphocytes in SV40 Infections -- Detection of SV40 DNA Sequences in Human Tissue -- Guide to Techniques in Creating Transgenic Mouse Models Using SV40 T Antigen.
520			_aExtensive research has shown that Simian Virus 40, a contaminant of polio and adenovirus vaccines that may be implicated in human cancers, can also serve as a powerful probe for examining many fundamental questions in molecular biology. In SV40 Protocols, Leda Raptis and a panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and its related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for transgenic mouse models based on the SV40 large T antigen. All methods have been optimized for experimental success, and the authors provide cogent discussions of the problems and pitfalls that may be encountered, as well as valuable troubleshooting advice. An appendix lists all companies whose products are cited in the text and includes an Internet directory for locating other reagent sources. Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors.
988			_aSpringer_Protocols_2001
650		7	_2embne _9140445 _aVirus
776	0	8	_iPrinted edition: _z9781489942074
776	0	8	_iPrinted edition: _z9780896036536
776	0	8	_iPrinted edition: _z9781489942067
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591175 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI