000			03978nam a22003975i 4500
999			_c392682 _d392682
001			392682
003			ES-MaUEC
005			20240113194049.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			101212s2011 xxu| o |||| 0|eng d
020			_a9781607619505
024	7		_a10.1007/978-1-60761-950-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH211 _b2011 EB
245	0	0	_aLight Microscopy : _bMethods and Protocols _cedited by Hélio Chiarini-Garcia, Rossana C. N. Melo
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (X, 204 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v689
505	0		_aGlycol methacrylate embedding for improved morphological, morphometrical and immunohistochemical investigations under light microscopy: testes as a model -- Histological processing of teeth and periodontal tissues for light microscopy analysis -- Large plant samples: how to process for 2-hydroxyethyl methacrylate embedding? -- Image cytometry: nuclear and chromosomal DNA quantification -- Histological approaches to study tissue parasitism during Trypanosoma cruzi infection -- Intravital microscopy to study leukocyte recruitment in vivo -- Introduction to fluorescence microscopy -- Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals -- Imaging lipid bodies within leukocytes with different light microscopy techniques -- EicosaCell - An immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites -- Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer -- Imaging calcium sparks in cardiac myocytes -- Light microscopy in aquatic ecology: methods for plankton communities studies -- Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedding samples.
520			_aOf all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.
988			_aSpringer_Protocols_2011
650		7	_2embne _9138719 _aMicroscopía
776	0	8	_iPrinted edition: _z9781607619499
776	0	8	_iPrinted edition: _z9781607619512
776	0	8	_iPrinted edition: _z9781493957132
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-950-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2023 _dz _eIG _zSI