000			05240nam a22004095i 4500
999			_c392680 _d392680 _x1
001			392680
003			ES-MaUEC
005			20240314174251.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			110729s2011 xxu| o |||| 0|eng d
020			_a9781617792106
024	7		_a10.1007/978-1-61779-210-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQL959 _b2011 EB
245	0	0	_aVertebrate Embryogenesis : _bEmbryological, Cellular, and Genetic Methods _cedited by Francisco J. Pelegri
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 605 páginas) _b92 ilustraciones, 9 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v770
505	0		_aApproaches and Species in the History of Vertebrate Embryology -- Manipulating and Imaging the Early Xenopus laevis Embryo -- Manipulation of Gene Function in Xenopus laevis -- Developmental Genetics in Xenopus tropicalis -- Embryological and Genetic Manipulation of Chick Development -- Embryological Manipulations in Zebrafish -- Practical Approaches for Implementing Forward Genetic Strategies in Zebrafish -- Essential Techniques for Introducing Medaka to a Zebrafish Laboratory - Toward the Combined Use of Medaka and Zebrafish for Further Genetic Dissection of the Function of the Vertebrate Genome -- Ex-utero Culture and Imaging of Mouse Embryos -- Detection of Gene Expression in Mouse Embryos and Tissue Sections -- Gene Targeting in the Mouse -- Creating a "Hopeful Monster": Mouse Forward Genetic Screens -- Assisted Reproductive Technology in Nonhuman Primates -- Embryological Methods in Ascidians: the Villefranche-sur-Mer Protocols -- Ciona Genetics -- Analyses of Gene Function in Amphioxus Embryos by Microinjection of mRNAs and Morpholino-oligonucleotides -- Reptile Embryology -- Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii -- Mutant Generation in Vertebrate Model Organisms by TILLING -- Inducing High Rates of Targeted Mutagenesis in Zebrafish using Zinc Finger Nucleases (ZFNs) -- Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic Cell Nuclear Transfer -- Cloning Mice and ES Cells by Nuclear Transfer from Somatic Cells and Fully Differentiated Cells -- Keeping Two Animal Systems in One Lab: A Frog Plus Fish Case Study -- Laboratory Guidelines for Animal Care.
520			_aOne of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species. Such conservation allows information learned from a given organism to be applicable to other species, including humans, and has validated the use of a few model systems to deduce general biological principles. In spite of this underlying conservation, however, each species has unique characteristics arising from its evolutionary history. Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species. In fact, this volume has been designed so that readers can readily find information on species other than the one with which they may be most familiar. These protocols include not only embryological methods, but also cellular and genetic approaches that have complemented and expanded our understanding of embryonic development.  In addition, a number of chapters highlight a specific method that is in principle applicable to multiple species, such as TILLING and ZFN-mediated mutagenesis, the generation of Embryonic Stem (ES) cell lines, and nuclear/oocyte transfer.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods serves as an ideal guide to the molecular, cell, and developmental biology community and will hopefully contribute to the ongoing collective effort towards a better understanding of the beauty and logic of vertebrate development.
988			_aSpringer_Protocols_2011
650		7	_2embne _9138502 _aVertebrados
650		7	_2embne _9679112 _aEmbriología veterinaria
776	0	8	_iPrinted edition: _z9781617792090
776	0	8	_iPrinted edition: _z9781617792113
776	0	8	_iPrinted edition: _z9781493961610
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-210-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI