000			04837nam a22003975i 4500
999			_c392673 _d392673 _x1
001			392673
003			ES-MaUEC
005			20240314174249.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s2004 xxu| o |||| 0|eng d
020			_a9781592594061
024	7		_a10.1385/1592594069 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC267 _b2004 EB
245	0	0	_aCancer Cell Culture : _bMethods and Protocols _cedited by Simon P. Langdon
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XII, 360 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v88
505	0		_ato Cancer Cell Culture -- Basic Principles of Cancer Cell Culture -- Essential Techniques of Cancer Cell Culture -- Characterization and Authentication -- Characterization and Authentication of Cancer Cell Lines -- Authentication of Cancer Cell Lines by DNA Fingerprinting -- Cytogenetic Characterization of Tumor Cell Lines -- Isolation and Culture of Specialized Cell Types -- Isolation and Culture of Colon Cancer Cell Lines -- Isolation and Culture of Melanoma Cell Lines -- Isolation and Culture of Human Brain Tumor Cells -- Isolation and Culture of Renal Cancer Cell Lines -- Isolation and Culture of Prostate Cancer Cell Lines -- Isolation and Culture of Ovarian Cancer Cell Lines -- Isolation and Culture of Leukemia Cell Lines -- Functional Assays in Cell Culture -- Cell Sensitivity Assays: Clonogenic Assay -- Cell Sensitivity Assays: The MTT Assay -- PARP Cleavage as a Means of Assessing Apoptosis -- Detection of Apoptosis by the TUNEL Assay -- Apoptosis Measurement by Annexin V Staining -- Cell Adhesion Assays -- Radial Monolayer Cell Migration Assay -- Invasion and Motility Assays -- Identification of Senescence in Cancer Cells -- Angiogenesis Assays -- Flow Cytometric DNA Analysis of Human Cancer Cell Lines -- Modification of Cancer Cells -- DNA-Mediated Gene Transfer -- Development of Drug-Resistant Models -- Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene -- The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts -- Coculture Systems -- Coculture of Prostate Cancer Cells -- Coculture of Ovarian Cells Using Porous Tissue Culture Inserts -- Cell Line Contamination -- Cell Culture Contamination -- Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction -- Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics.
520			_aThe growing of cancer cells in the laboratory has been a powerful tool for studying and understanding the biology of cancer and the effects of drugs on cancer cells. In Cancer Cell Culture: Methods and Protocols, expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. Suitable for novice and experienced researchers alike, these readily reproducible techniques solve a diverse range of experimental problems. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the coculture of different cell types; and the detection and treatment of contamination. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Cancer Cell Culture: Methods and Protocols provides both basic scientists and clinical researchers with a gold-standard collection of advanced techniques for culturing cancer cells effectively in their laboratories.
988			_aSpringer_Protocols_2004
650		7	_2embne _9146156 _aCélulas cancerosas
776	0	8	_iPrinted edition: _z9781617373435
776	0	8	_iPrinted edition: _z9781588290793
776	0	8	_iPrinted edition: _z9781489939388
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592594069 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI