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008			100301s2009 xxu| o |||| 0|eng d
020			_a9781603275651
024	7		_a10.1007/978-1-60327-565-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR342 _b2009 EB
245	0	0	_aBacteriophages : _bMethods and Protocols, Volume 2: Molecular and Applied Aspects _cedited by Martha R. J. Clokie, Andrew Kropinski
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XXII, 373 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v502
505	0		_aBacteriophage Genomics -- Preparation of Bacteriophage Lysates and Pure DNA -- Approaches to the Compositional Analysis of DNA -- Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis -- Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing -- PCR and Partial Sequencing of Bacteriophage Genomes -- In Sffamily Identification of Genes in Bacteriophage DNA -- Determining DNA Packaging Strategy by Analysis of the Termini of the Chromosomes in Tailed-Bacteriophage Virions -- In silico Characterization of DNA Motifs with Particular Reference to Promoters and Terminators -- Molecular Phylogenetics: Testing Evolutionary Hypotheses -- Bacteriophage Transcriptomics and Proteomics -- Preparation of RNA from Bacteria Infected with Bacteriophages: A Case Study from the Marine Unicellular Synechococcus sp. WH7803 Infected by Phage S-PM2 -- Quantification of Host and Phage mRNA Expression During Infection Using Real-Time PCR -- Oligonucleotide Microarrays for Bacteriophage Expression Studies -- Purification of Bacteriophages and SDS-PAGE Analysis of Phage Structural Proteins from Ghost Particles -- Phage Proteomics: Applications of Mass Spectrometry -- Community Bacteriophage Approaches -- Isolation Independent Methods of Characterizing Phage Communities 1: Strain Typing Using Fingerprinting Methods -- Isolation Independent Methods of Characterizing Phage Communities 2: Characterizing a Metagenome -- Applied Aspects Of Bacteriophage Biology -- Phage Typing -- A Genetic Screen to Identify Bacteriophage Lysins -- General M13 Phage Display: M13 Phage Display in Identification and Characterization of Protein-Protein Interactions -- Isolation of Monoclonal Antibody Fragments from Phage Display Libraries -- Internet Resources of Interest to Bacteriophage Workers.
520			_aRanging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.
988			_aSpringer_Protocols_2009
650		7	_2embne _9671714 _aBacteriófagos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607611066
776	0	8	_iPrinted edition: _z9781617379109
776	0	8	_iPrinted edition: _z9781603275644
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-565-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b12/2023 _dz _eIG _zSI