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020 _a9781592595389
024 7 _a10.1385/0896032752
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aTP248.65 .I49
_b1995 EB
245 0 0 _aAntibody Engineering Protocols
_cedited by Sudhir Paul
250 _a1st edition 1995
264 1 _aTotowa, NJ
_bHumana Press
_c1995
300 _a1 recurso en línea (XII, 450 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v51
505 0 _aSEQHUNT -- Molecular Modeling of Antibody-Combining Sites -- Structure and Properties of Human Immunoglobulin Light-Chain Dimers -- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins -- Detection of Human Variable Gene Family Expression at the Single-Cell Level -- Purification of Reduced and Alkylated Antibody Subunits -- Murine Monoclonal Antibody Development -- Comparative Properties of Polyclonal and Monoclonal Antibodies -- Evaluation of Antibody Clonality -- Affinity Immunoblotting -- Epitope and Idiotope Mapping Using Monoclonal Antibodies -- Anti-Idiotypic Antibodies That Mimic Opioids -- Catalytic Antibodies -- Preparation and Assay of Acetylcholinesterase Antibody -- DNA Hydrolysis by Antibodies -- Screening Strategies for Catalytic Antitransition-State Analog Antibodies -- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells -- Single-Chain Anti-DNA Fv -- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments -- Cloning and Bacterial Expression of an Esterolytic sFv -- Site-Directed Mutagenesis of Antibody-Variable Regions -- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation -- Chaperonins in Phage Display of Antibody Fragments -- Phage-Display Libraries of Murine and Human Antibody Fab Fragments -- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library -- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography -- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening -- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies -- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies -- Radiolabeling of Antibodies for Therapy and Diagnosis.
988 _aSpringer_Protocols_1995
650 7 _2embne
_9143641
_aInmunoglobulinas
776 0 8 _iPrinted edition:
_z9781489940742
776 0 8 _iPrinted edition:
_z9780896032750
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032752
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eIG
_zSI