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008			161104s2017 xxu| o |||| 0|eng d
020			_a9781493965816
024	7		_a10.1007/978-1-4939-6581-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR187 .P4 _b2017 EB
245	0	0	_aPhagocytosis and Phagosomes : _bMethods and Protocols _cedited by Roberto Botelho.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 360 páginas) _b57 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1519
505	0		_aPhagocytosis: Hungry, Hungry Cells -- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry -- Quantitative Efferocytosis Assays -- Quantifying Phagocytosis by Immunofluorescence and Microscopy -- Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage and Cell Death Following Exposure of Macrophages to Silica Particles -- Quantitative Live-cell Fluorescence Microscopy during Phagocytosis -- Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers -- Quantitative Immunofluorescence to Study Phagosome Maturation -- Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho Gtpase Activation during Phagocytosis -- Analysis of LC3-associated Phagocytosis and Antigen Presentation -- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells -- Measuring Phagosomal pH by Fluorescence Microscopy -- Image-Based Analysis of Phagocytosis - Measuring Engulfment and Internalization -- Fluorometric Approaches to Measuring Reductive and Oxidative Events in Phagosomes -- Simultaneous Analysis of Multiple Lumenal Parameters of Individual Phagosomes Using High-Content Imaging -- <isolation and="" western="" blotting="" of="" latex-bead="" phagosomes="" to="" track="" phagosome="" maturation.-="" assessing="" the="" proteome="" by="" quantitative="" mass="" spectrometry.-="" dissecting="" phagocytic="" removal="" apoptotic="" cells="" in="" Caenorhabditis Elegans -- Measurement of Salmonella Enterica Internalization and Vacuole Lysis in Epithelial Cells -- Bacterial Binding, Phagocytosis and Killing: Measurements Using Colony Forming Units -- Filamentous Bacteria as Targets to Study Phagocytosis -- Growing and Handling of Mycobacterium Tuberculosis for Macrophage Infection Assays -- Mycobacterium Tuberculosis: Readouts of Bacterial Fitness and The Environment within the Phagosome -- Using Flow Cytometry to Analyse Cryptococcus Infection in Macrophages.>.
520			_aThis volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.
988			_aSpringer_Protocols_2017
650		7	_2embne _9674677 _aFagocitosis _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493965793
776	0	8	_iPrinted edition: _z9781493965809
776	0	8	_iPrinted edition: _z9781493982417
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6581-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eu _zSI