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005			20231106145140.0
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008			110429s2011 xxu| o |||| 0|eng d
020			_a9781617791239
024	7		_a10.1007/978-1-61779-123-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981 _b2011 EB
245	0	0	_aRNAi and Plant Gene Function Analysis : _bMethods and Protocols _cedited by Hiroaki Kodama, Atsushi Komamine
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (X, 244 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v744
505	0		_aOverview of Plant RNAi -- Caveat of RNAi in Plants: the Off-Target Effect -- Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies -- Heat-Inducible RNAi for Gene Functional Analysis in Plants -- Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens -- Virus-Induced Gene Silencing in Ornamental Plants -- Local RNA Silencing Mediated by Agroinfiltration -- Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of Arabidopsis thaliana -- Detection of Long and Short Double-Stranded RNAs -- Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs -- Large Scale Sequencing of Plant Small RNAs -- Computational Prediction of Plant miRNA Targets -- Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants -- Using Nuclear Run-On Transcription Assays in RNAi Studies -- Proteomic Analysis of RNA-Interference Induced Knockdown Plant -- Comparative Analysis of Phosphoprotein Expression using 2D-DIGE.
520			_aThe use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.
988			_aSpringer_Protocols_2011
650		7	_2embne _9139640 _aGenética vegetal _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617791222
776	0	8	_iPrinted edition: _z9781627039321
776	0	8	_iPrinted edition: _z9781617791246
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-123-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eu _zSI