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008			100301s1997 xxu| o |||| 0|eng d
020			_a9781592595648
024	7		_a10.1385/0896034089 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRS431 .P37 _b1997 EB
245	0	0	_aAntibacterial Peptide Protocols _cedited by William Shafer
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (X, 259 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v78
505	0		_aOrigins and Development of Peptide Antibiotic Research -- Origins and Development of Peptide Antibiotic Research -- Isolation and Characterization of Antibacterial Peptides -- HPLC Methods for Purification of Antimicrobial Peptides -- Strategies for the Isolation and Characterization of Antimicrobial Peptides of Invertebrates -- Big Defensin and Tachylectins-1 and -2 -- Tachyplesin and Anti-Lipopolysaccharide Factor -- Circular Dichroism Studies of Secondary Structure of Peptides -- Analytical Ultracentrifugation Studies of Association of Peptides -- NMR Characterization of Amphipathic Helical Peptides -- Molecular Biology of Antibacterial Peptides -- Laboratory Production of Antimicrobial Peptides in Native Conformation -- An Approach Combining Rapid cDNA Amplification and Chemical Synthesis for the Identification of Novel, Cathelicidin-Derived, Antimicrobial Peptides -- Molecular Biological Strategies in the Analysis of Antibiotic Peptide Gene Families -- Assay Systems for Studying Antibacterial Peptides -- Designer Assays for Antimicrobial Peptides -- Interaction of Cationic Peptides with Bacterial Membranes -- The Genetic Basis of Microbial Resistance to Antimicrobial Peptides -- Assay of Antibacterial Activities of the Bactericidal/Permeability-Increasing Protein in Natural Biological Fluids -- Assay Systems for Measurement of Chemotactic Activity -- Neutralization of the In Vivo Activity of E. Coli-Derived Lipopolysaccharide by Cationic Peptides.
520			_aIn Antibacterial Peptide Protocols, leading authorities review for the first time in one volume all the major biochemical, molecular, bacteriological, and physical techniques available to assess antimicrobial peptides. These state-of-the-art methods ensure easily reproducible results in such important procedures as the isolation and characterization of antimicrobial peptides, the molecular characterization of genes encoding antimicrobial peptides, and the use of expression systems to isolate peptides. Bioassays and microbial genetic techniques are also included, as are antibacterial assays as the final readout system. These methods detailed in Antibacterial Peptide Protocols will play an important role in the treatment of infectious diseases, particularly with the increasing problem of multidrug-resistant microbes and the relative dearth of new antibiotics being provided by pharmaceutical companies.
988			_aSpringer_Protocols_1997
650		7	_2embne _9146243 _aPéptidos _xInvestigación _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617370274
776	0	8	_iPrinted edition: _z9780896034082
776	0	8	_iPrinted edition: _z9781489942869
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034089 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eu _zSI