000			04591nam a22003975i 4500
999			_c392643 _d392643
001			392643
003			ES-MaUEC
005			20231103143707.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			160519s2016 xxu| o |||| 0|eng d
020			_a9781493935420
024	7		_a10.1007/978-1-4939-3542-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH605 _b2016 EB
245	0	4	_aThe Mitotic Spindle : _bMethods and Protocols _cedited by Paul Chang, Ryoma Ohi
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XIII, 424 páginas) _b79 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1413
505	0		_aUsing Fluorescence Microscopy to Study Mitosis -- Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation -- Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts -- Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro -- Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles -- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy -- Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size -- In Vitro Kinetochore Assembly -- Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes -- Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells -- Studying Kinetochores In Vivo Using FLIM-FRET -- Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies -- A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion -- Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation -- Analyzing Spindle Positioning Dynamics in Cultured Cells -- Quantification of Mitotic Chromosome Alignment -- Imaging Mitosis in the Moss Physcomitrella Patens -- Small Molecule Approach to Study the Function of Mitotic Kinesins -- Identification and Characterization of Mitotic Spindle-Localized Transcripts -- Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization -- Studying Kinetochore Kinases -- Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics -- Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer -- Generation and Purification of Tetraploid Cells -- Anti-Microtubule Drugs.
520			_aThis volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field. .
988			_aSpringer_Protocols_2016
650		7	_2embne _9139477 _aCitología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493935406
776	0	8	_iPrinted edition: _z9781493935413
776	0	8	_iPrinted edition: _z9781493980727
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3542-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eu _zSI