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008			100301s2003 xxu| o |||| 0|eng d
020			_a9781592593002
024	7		_a10.1385/1592593003 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH441.5 _b2003 EB
245	0	0	_aMolecular Cytogenetics : _bProtocols and Applications _cedited by Yao-Shan Fan
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (X, 414 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v204
505	0		_aBasic Concepts and Techniques -- Molecular Cytogenetics in Medicine -- Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets -- Labeling Fluorescence In Situ Hybridization Probes for RNA Targets -- Basic FISH Techniques and Troubleshooting -- Evolving Techniques and Applications -- Chromosome Microdissection -- Primed In Situ Labeling -- Spectral Karyotyping -- Multicolor FISH -- Characterization of Constitutional Chromosome Abnormalities by Comparative Genomic Hybridization -- Color Banding -- Multicolor Fiber FISH -- Multi-Telomere FISH -- Fluorescence Genotyping for Screening Cryptic Telomeric Rearrangements -- Microarray CGH -- Special Applications in Chromosomal Disorders -- Molecular Approaches for Delineating Marker Chromosomes -- Interphase FISH for Prenatal Diagnosis of Common Aneuploidies -- Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood -- Preimplantation FISH Diagnosis of Aneuploidies -- Microdeletion Syndromes -- Molecular Detection of Uniparental Disomy -- Molecular Cytogenetics in Reproductive Pathology -- Special Applications in Oncology -- Interphase FISH Studies of Chronic Myeloid Leukemia -- Chromogenic In Situ Hybridization and FISH in Pathology -- FISH Detection of HER2 Amplification in Breast Cancer -- Comparative Genomic Hybridization in Cancer Investigations -- Simultaneous Fluorescence Immunophenotyping and FISH on Tumor Cells -- BAC Resource for Molecular Cytogenetics.
520			_aWith the proliferation of fluorescence in situ hybridization (FISH) techniques as a powerful diagnostic tool in many areas of medicine, the variety and complexity of tests pose bewildering choices even for long-experienced laboratory technologists and physicians. In Molecular Cytogenetics: Protocols and Applications, Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step fashion not only the leading FISH techniques, but also those molecular technologies beyond FISH now available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics. Each easy-to-follow protocol offers first hand advice on how to obtain the best results, to avoid pitfalls, and to troubleshoot problems. Many protocols discuss possible future applications of the technology and explain its sensitivity and limitations. Cutting-edge and highly practical, Molecular Cytogenetics: Protocols and Applications offers laboratory physicians and scientists today's most comprehensive and up-to-date collection of FISH protocols for cancer diagnosis and investigation, plus an array of the emerging molecular technologies beyond FISH.
988			_aSpringer_Protocols_2003
650		7	_2embne _9139622 _aCitogenética _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617373008
776	0	8	_iPrinted edition: _z9781588290069
776	0	8	_iPrinted edition: _z9781489938589
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593003 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _eu _zSI