000			03970nam a22003975i 4500
999			_c392634 _d392634
001			392634
003			ES-MaUEC
005			20231026142537.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			130804s2013 xxu| o |||| 0|eng d
020			_a9781627035323
024	7		_a10.1007/978-1-62703-532-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_b2013 EB
245	0	0	_aG Protein-Coupled Receptor Signaling in Plants : _bMethods and Protocols _cedited by Mark P. Running
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 162 páginas) _b19 ilustraciones, 14 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1043
505	0		_aTopology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs -- Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins -- Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana -- Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants -- Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis -- Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis -- Fusarium oxysporum Infection Assays in Arabidopsis -- Analysis of Unfolded Protein Response in Arabidopsis -- Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis -- G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings -- Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana -- In Vivo Localization in Arabidopsis Protoplasts and Root Tissue -- Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography-Coupled Mass Spectrometry -- In Vitro Myristoylation Assay of Arabidopsis Proteins -- Assaying Protein S-Acylation in PlantIn Vitro Prenylation Assay of Arabidopsis Proteins.
520			_aRemarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins.  Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.
988			_aSpringer_Protocols_2013
650		7	_2embne _9146302 _aCélulas vegetales _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627035316
776	0	8	_iPrinted edition: _z9781627035330
776	0	8	_iPrinted edition: _z9781493962167
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-532-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eu _zSI