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008			100301s1999 xxu| o |||| 0|eng d
020			_a9781592596034
024	7		_a10.1385/0896035514 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR396 _b1999 EB
245	0	0	_aAdenovirus Methods and Protocols _cedited by William S. M. Wold
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XIII, 352 páginas) _b72 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v21
505	0		_aPreparation and Titration of CsCl-Banded Adenovirus Stock -- Construction of Mutations in the Adenovirus Early Region 3 (E3) Transcription Units -- Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants -- Manipulation of Early Region 4 -- Adenovirus DNA Packaging -- Methods for Creating and Analyzing Adenovirus Vectors that Express Proteins that Act on the Viral Genome -- Construction of Mouse Adenovirus Type 1 Mutants -- Generation of Adenovirus-Specific Cytotoxic T Lymphocytes -- Measurement of Macrophage-Mediated Cytolysis of Adenovirus-Infected Cells -- Measurement of Tumor Necrosis Factor (TNF) Lysis of Adenovirus-Infected Cells -- Role of Natural Killer (NK) Cells in Immunity to Adenoviruses -- Determination of the Transforming Activities of Adenovirus Oncogenes -- Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members -- In Vitro Transcription -- Cell Microinjection -- Immunoprecipitation of E1 A-Containing Protein Complexes -- Preparation of Splicing-Competent Nuclear Extracts from Adenovirus-lnfected Cells -- Adenovirus Entry into Cells -- Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunostaining -- Characterization of the Adenovirus Fiber Protein -- Large-Scale Purification and Crystallization of Adenovirus Hexon -- Adenovirus Protease -- Methods for Growth and Purification of Enteric Adenovirus Type 40 -- Transfection Complexes Generated with Adenovirus and Polyethylenimine-Condensed DNA -- Phylogenetic Analysis of Adenovirus Sequences.
520			_aIn Adenovirus Methods and Protocols, William S.M. Wold has organized a collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state. Each method is written by a prominent investigator well-versed in the technique and includes a brief background discussion, as well as tried and true step-by-step instructions. Adenovirus Methods and Protocols will be useful to both entry-level and senior scientists seeking to enter the adenovirus field, to researchers from other areas wishing to construct adenovirus vectors for their own research, and to adenovirologists wanting to enter new sectors of research. Its cutting-edge techniques are certain to make it today's reference of choice, one from which even expert researchers will learn many productive and time-saving techniques.
988			_aSpringer_Protocols_1999
650		7	_2embne _9140445 _aVirus _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781489943453
776	0	8	_iPrinted edition: _z9781489943446
776	0	8	_iPrinted edition: _z9780896035515
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896035514 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eu _zSI