000			04373nam a22003855i 4500
999			_c392629 _d392629
001			392629
003			ES-MaUEC
005			20240122120722.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			100301s2007 xxu| o |||| 0|eng d
020			_a9781597453998
024	7		_a10.1007/978-1-59745-399-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.2 _b2007 EB
245	0	0	_aAnimal Cell Biotechnology : _bMethods and Protocols _cedited by Ralf Pörtner
250			_a2nd edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XV, 512 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Biotechnology _x1940-607X _v24
505	0		_aBasic Techniques for Establishing Production Cell Lines -- Transduction Technologies -- Cells for Gene Therapy and Vector Production -- Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments -- DNA Fingerprinting and Characterization of Animal Cell Lines -- Basic Cultivation Techniques -- Microcarrier Cell Culture Technology -- Cell Encapsulation -- Tools for High-Throughput Medium and Process Optimization -- Cell Characterization and Analysis -- Cell Counting and Viability Measurements -- Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry -- Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors -- Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture -- Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation -- Measurement of Apoptosis in Cell Culture -- Metabolic Flux Estimation in Mammalian Cell Cultures -- Special Cultivation Techniques -- Disposable Bioreactors for Inoculum Production and Protein Expression -- Hollow Fiber Cell Culture -- Cultivation of Mammalian Cells in Fixed-Bed Reactors -- Configuration of Bioreactors -- Downstream Techniques -- Membrane Filtration in Animal Cell Culture -- Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins -- Special Applications -- Vaccine Production -- Retrovirus Production and Characterization -- Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production.
520			_aAnimal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors. Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
988			_aSpringer_Protocols_2007
650		7	_2embne _9140931 _aBiotecnología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781588296603
776	0	8	_iPrinted edition: _z9781627039635
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-399-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eu _zSI