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008			150311s2015 xxu| o |||| 0|eng d
020			_a9781493924448
024	7		_a10.1007/978-1-4939-2444-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981 _b2015 EB
245	0	0	_aPlant Functional Genomics : _bMethods and Protocols _cedited by Jose M. Alonso, Anna N. Stepanova
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XV, 532 páginas) _b85 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1284
505	0		_aEpigenome Profiling of Specific Plant Cell Types Using a Streamlined INTACT Protocol and ChIP-seq -- Whole-Genome DNA Methylation Profiling with Nucleotide Resolution -- High-Throughput Nuclease-Mediated Probing of RNA Secondary Structure in Plant Transcriptomes -- Genome-Wide Mapping of DNase I Hypersensitive Sites in Plants -- Characterization of in-vivo DNA-Binding Events of Plant Transcription Factors by ChIP-seq: Experimental Protocol and Computational Analysis -- Identification of Direct Targets of Plant Transcription Factors Using the GR Fusion Technique -- Ribosome Profiling: A Tool for Quantitative Evaluation of Dynamics in mRNA Translation -- Tissue-Specific Gene Expression Profiling by Cell Sorting -- Translating Ribosome Affinity Purification (TRAP) Followed by RNA Sequencing Technology (TRAP-SEQ) for Quantitative Assessment of Plant Translatomes -- Rapid Immunopurification of Ribonucleoprotein Complexes of Plants -- Metabolomic Profiling of Plant Tissues -- Targeted Plant Genome Editing via the CRISPR/Cas9 Technology -- QTL Mapping Using High-Throughput Sequencing -- Quantitating Plant MicroRNA-Mediated Target Repression Using a Dual-Luciferase Transient Expression System -- Persistent Virus-Induced Gene Silencing in Asymptomatic Accessions of Arabidopsis -- A User's Guide to the Arabidopsis T-DNA Insertion Mutant Collections -- Genome-Wide Association Mapping in Plants Exemplified for Root Growth in Arabidopsis thaliana -- Tilling By Sequencing -- SHOREmap v3.0: Fast and Accurate Identification of Causal Mutations from Forward Genetic Screens -- Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework -- Ligation Independent Cloning for Plant Research -- Gene Functional Analysis Using Protoplast Transient Assays -- Descriptive vs. Mechanistic Network Models in Plant Development in the Post-Genomic Era -- Analysis and Visualization of RNA-Seq Expression Data Using RStudio, Bioconductor, and Integrated Genome Browser -- Constructing Simple Biological Networks for Understanding Complex High-Throughput Data in Plants.
520			_aThis second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene's function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates, and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements, and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.
988			_aSpringer_Protocols_2015
650		7	_2embne _9672192 _aBiología molecular vegetal _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493924431
776	0	8	_iPrinted edition: _z9781493924455
776	0	8	_iPrinted edition: _z9781493949137
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2444-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eu _zSI