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003			ES-MaUEC
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008			130220s2013 xxu| fo |||| 0|eng d
020			_a9781627032933
024	7		_a10.1007/978-1-62703-293-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP601 _b2013 EB
245	0	0	_aEnzyme Engineering : _bMethods and Protocols _cedited by James C. Samuelson
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 252 páginas) _b52 ilustraciones, 19 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v978
505	0		_aA Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivo -- Determining Enzyme Kinetics via Isothermal Titration Calorimetry -- GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria -- Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage Properties -- TAL Effector Nuclease (TALEN) Engineering -- In vitro Evolution of Enzymes -- Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivo -- Reconstructing Evolutionary Adaptive Paths for Protein Engineering -- Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria -- FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants -- Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes -- Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides -- Protein Engineering: Single or Multiple Site-Directed Mutagenesis -- Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination -- Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments -- Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis -- Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter -- In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates.
520			_aWhether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.
988			_aSpringer_Protocols_2013
650		7	_2embne _9138640 _aEnzimas _xBiotecnología
776	0	8	_iPrinted edition: _z9781627032940
776	0	8	_iPrinted edition: _z9781627032926
776	0	8	_iPrinted edition: _z9781493962877
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-293-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI