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008			100715s2009 xxu| fo |||| 0|eng d
020			_a9781607612322
024	7		_a10.1007/978-1-60761-232-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH430 _b2009 EB
245	0	0	_aReverse Chemical Genetics : _bMethods and Protocols _cedited by Hisashi Koga
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XII, 272 páginas) _b64 ilustraciones, 4 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v577
505	0		_aThe Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics -- ORFeome Cloning -- Systematic Cloning of an ORFeome Using the Gateway System -- High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins -- Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study -- High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data -- Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology -- Modification of cDNA Resources Utilized for Reverse Chemical Genetics -- High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources -- An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources -- Recombinant Protein Production by a Kaiko-Baculovirus System -- Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins -- The Refinement of Established Methods by Recent Innovations -- Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine -- Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis -- Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos -- Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins -- Potential Applicable Techniques for Reverse Chemical Genetics -- Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases -- Preparation of Highly Sensitive Protein Array Using Reactive Polymer -- High-Throughput SPR Biosensor -- Drug Discovery Through Functional Screening in the Drosophila Heart -- Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic Activity -- Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety.
520			_aNow a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.
988			_aSpringer_Protocols_2009
650		7	_2embne _9138639 _aBioquímica
776	0	8	_iPrinted edition: _z9781607612339
776	0	8	_iPrinted edition: _z9781607612315
776	0	8	_iPrinted edition: _z9781617796418
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-232-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI