000			03726nam a22003975i 4500
999			_c392588 _d392588
001			392588
003			ES-MaUEC
005			20231202124939.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			100301s2003 xxu| s |||| 0|eng d
020			_a9781592593217
024	7		_a10.1385/1592593216 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR201 .H22 _b2003 EB
245	0	0	_aHaemophilus influenzae Protocols _cedited by Mark A. Herbert, Derek W. Hood, E. Richard Moxon
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XI, 333 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v71
505	0		_aThe Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae -- The Pathogenesis of Disease Due to Type b Haemophilus influenzae -- General Methods for Culturing Haemophilus influenzae -- Transformation of Haemophilus influenzae -- Diagnosis of Infection -- Characterization of Plasmids -- Protein Characterization by Two-Dimensional Gel Electrophoresis -- Monitoring Gene Expression Using DNA Arrays -- Gene Expression Technology -- The Genome Sequence of Haemophilus influenzae -- Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae -- Mutagenesis of H. influenzae -- Transposon Tn 10 -- In Vivo Expression of Bacterial Genes During Human Infections -- ELISA -- Opsonophagocytosis Assay Using Flow-Cytometry -- In Vitro Models of Infection I-Human Respiratory Tissue Organ Culture -- In Vitro Models of Infection II-Human Umbilical Vein Endothelial Cells (HUVECs) System -- Animal Models.
520			_aAs the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models. Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection of powerful molecular methods that illuminate not only how the bacterium causes disease, but also how best to develop much-needed new vaccines and antibiotics against it.
988			_aSpringer_Protocols_2003
650		7	_2embne _9138901 _aBacterias
776	0	8	_iPrinted edition: _z9781617372551
776	0	8	_iPrinted edition: _z9780896039285
776	0	8	_iPrinted edition: _z9781489928139
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593216 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI