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020 _a9781592592432
024 7 _a10.1385/1592592430
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK898.C5
_b2002 EB
245 0 0 _aHeme, Chlorophyll, and Bilins :
_bMethods and Protocols
_cedited by Alison Smith, Michael Witty
250 _a1st edition 2002
264 1 _aTotowa, NJ
_bHumana Press
_c2002
300 _a1 recurso en línea (IX, 340 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aSpringer Protocols Handbooks
_x1949-2456
505 0 _aLaboratory Methods for the Study of Tetrapyrroles -- Syntheses of Tetrapyrroles -- General Laboratory Methods for Tetrapyrroles -- Enzymatic Preparation of Tetrapyrrole Intermediates -- Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme -- Analysis of Intermediates and End Products of the Chlorophyll Biosynthetic Pathway -- Analysis of Heme and Hemoproteins -- Hemoproteins Purification and Characterization by Using Aqueous Two-Phase Systems -- Structural Study of Heme Proteins by Electron Microscopy of 2-Dimensional Crystals -- Analysis and Reconstitution of Chlorophyll-Proteins -- Two-Dimensional Crystallization of Chlorophyll Proteins -- Biosynthesis and Analysis of Bilins -- Analysis and Reconstitution of Phytochromes -- Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions.
520 _aAlthough researchers can profitably investigate heme, chlorophyll, and related tetrapyrroles in a wide range of academic and medical research programs, the handling and manipulation of these delicate compounds requires considerable skill and cross-boundary knowledge. In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators overcomes these limitations by describing in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles, heme, and hemoproteins, to the biosynthesis and the analysis of chlorophyll and bilins. Timely and highly practical, Heme, Chlorophyll, and Bilins: Methods and Protocols is a gold-standard collection of readily reproducible techniques suitable for a wide range of researchers, whether it be a clinician studying photodynamic therapy, an ecologist studying the chlorophyll composition of leaves in a tropical forest, or a cell biologist investigating the function of specific hemoproteins.
988 _aSpringer_Protocols_2002
650 7 _2embne
_9154388
_aClorofila
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781617373657
776 0 8 _iPrinted edition:
_z9781588291110
776 0 8 _iPrinted edition:
_z9781489938657
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592430
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_ean
_zSI