000			03308nam a22003975i 4500
999			_c392576 _d392576
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008			100301s2001 xxu| fo |||| 0|eng d
020			_a9781592590858
024	7		_a10.1385/1592590853 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRE58 _b2001 EB
245	0	0	_aVision Research Protocols _cedited by P. Elizabeth Rakoczy
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (X, 320 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v47
505	0		_aBasic Molecular Biology Techniques -- Transient Transfection of Human Retinoblastoma Cells -- In Situ Hybridization -- Immunocytochemical Studies of the Retina -- Antisense DNA Technology -- Inhibition of Gene Expression by Ribozymes -- Intraocular Delivery of Recombinant Virus -- Preparation of Recombinant Adenoviruses -- Generation of Recombinant Adeno-Associated Virus -- Preparation of Recombinant Retroviruses -- Generation of Transgenic Mice for Studies of Ocular Development and Disease -- Generation of Knockout Animal Models -- Mutation Analysis of Ocular Genes -- Ophthalmologists' Perspective.
520			_aMuch fascinating and valuable research has been devoted to the molecular biology of the eye, its various structures, and its innervation this past decade. In Vision Research Protocols, Elizabeth Rakoczy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the downregulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. The book details the three most popular viral delivery vehicles--recombinant adeno, adeno-associated, and retroviruses-as well as the major delivery methods for the most widely used animal models. Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of the most powerful tools with which to ask--and answer--all the important questions emerging from the dramatically advancing work on vision research today.
988			_aSpringer_Protocols_2001
650		7	_2embne _9139103 _aBiología molecular _vMetodología
776	0	8	_iPrinted edition: _z9781617372148
776	0	8	_iPrinted edition: _z9781489943750
776	0	8	_iPrinted edition: _z9780896038370
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590853 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI