000			04470nam a22003975i 4500
999			_c392572 _d392572 _x1
001			392572
003			ES-MaUEC
005			20231115145619.0
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007			cr nn 008mamaa
008			100301s2003 xxu| fo |||| 0|eng d
020			_a9781592593668
024	7		_a10.1385/1592593666 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP88.2 _b2003 EB
245	0	0	_aBone Research Protocols _cedited by Miep H. Helfrich, Stuart H. Ralston.
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XIV, 450 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v80
505	0		_aCulture of Osteolblasts and Osteocytes -- Human Osteoblast Culture -- Osteoblast Isolation from Murine Calvariae and Long Bones -- Mineralizing Fibroblast-Colony-Forming Assays -- Osteocyte Isolation and Culture -- Culture of Osteoclasts -- Isolated Osteoclast Cultures -- Primary Isolation and Culture of Chicken Osteoclasts -- Isolation and Purification of Rabbit Osteoclasts -- Generating Human Osteoclasts from Peripheral Blood -- Generating Human Osteoclasts In Vitro from Bone Marrow and Peripheral Blood -- Generating Murine Osteoclasts from Bone Marrow -- Osteoclast Formation in the Mouse Coculture Assay -- RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells -- Bone Organ Culture -- Analysis of Osteoclast Function in Mouse Calvarial Cultures -- Assessing Bone Formation Using Mouse Calvarial Organ Cultures -- Histological and Ultrastructural Techniques -- In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections -- Techniques for the Study of Apoptosis in Bone -- Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohistochemistry -- Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections -- Fluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal Microscopy -- Bone Histomorphometry -- Transmission Electron Microscopy of Bone -- Scanning Electron Microscopy of Bone -- Imaging Techniques -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- In Vivo Techniques -- Studies of Local Bone Remodeling -- Inflammation-Induced Osteoporosis -- Ovariectomy and Estrogen Replacement in Rodents -- Mechanical Loading Techniques -- Mechanical Testing of Bone Ex Vivo -- Bone Cell Responses to Fluid Flow -- Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models -- Molecular Analysis -- Extraction of Nucleic Acids from Bone -- Analysis of Gene Expression in Bone by Quantitative RT-PCR.
520			_aThe past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which these genes affect bone mass and strength. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone. Though in vitro methods receive central attention, there are also protocols for in vivo analyses of bone formation and resorption. State-of-the-art and highly practical, Bone Research Protocols offers both beginning and advanced laboratory researchers a major compendium of well-established techniques for studying bone cell function and metabolism.
988			_aSpringer_Protocols_2003
650		7	_2embne _9666561 _aHuesos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617373213
776	0	8	_iPrinted edition: _z9781588290441
776	0	8	_iPrinted edition: _z9781489938527
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593666 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean