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020 _a9781627030113
024 7 _a10.1007/978-1-62703-011-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH450
_b2012 EB
245 0 0 _aGenomic Imprinting :
_bMethods and Protocols
_cedited by Nora Engel
250 _a1st edition 2012
264 1 _aTotowa, NJ
_bHumana Press
_c2012
300 _a1 recurso en línea (XI, 297 páginas)
_b40 ilustraciones, 19 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v925
505 0 _aUniparental Embryos in the Study of Genomic Imprinting -- Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species -- Generation of Trophoblast Stem Cells -- Immunomagnetic Purification of Murine Primordial Germ Cells -- Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA -- Identification of Imprinted Loci by Transcriptome Sequencing -- Data Mining as a Discovery Tool for Imprinted Genes -- Engineering of Large Deletions and Duplications In Vivo -- Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells -- Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains -- Quantitative Chromosome Conformation Capture -- Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing -- Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies -- Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs -- Co-Immunoprecipitation of Long Non-Coding RNAs -- Specialized Technologies for Epigenetics in Plants -- Computational Studies of Imprinted Genes -- Insights on Imprinting from Beyond Mice and Men -- Non-Mammalian Parent-Of-Origin Effects.
520 _aGenomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting.  This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in  unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.
988 _aSpringer_Protocols_2012
650 7 _2embne
_9162770
_aExpresión génica
776 0 8 _iPrinted edition:
_z9781627030120
776 0 8 _iPrinted edition:
_z9781627030106
776 0 8 _iPrinted edition:
_z9781493959662
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-011-3
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_ean
_zSI