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008			140522s2014 xxu| fo |||| 0|eng d
020			_a9781493908448
024	7		_a10.1007/978-1-4939-0844-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK913 _b2014 EB
245	0	0	_aPlant Cold Acclimation : _bMethods and Protocols _cedited by Dirk K. Hincha, Ellen Zuther
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 282 páginas) _b75 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1166
505	0		_aMeasuring Freezing Tolerance: Survival and Regrowth Assays -- Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays -- Conducting Field Trials for Freezing Tolerance Breeding in Cereals -- A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance -- Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation -- Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic Regions -- Identification of Arabidopsis Mutants with Altered Freezing Tolerance -- Infrared Thermal Analysis of Plant Freezing Processes -- Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues -- Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images -- Proteomic Approaches to Identify Cold Regulated Soluble Proteins -- Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins -- Profiling Methods to Identify Cold Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry -- A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition -- Quantification of Superoxide and Hydrogen Peroxide in Leaves -- Estimating Ice Encasement Tolerance of Herbage Plants -- Characterization of Ice Binding Proteins from Sea Ice Algae -- Isolation and Characterization of Ice Binding Proteins from Higher Plants. .
520			_aPlant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties.  Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches.
988			_aSpringer_Protocols_2014
650		7	_2embne _9168918 _aPlantas _xAclimatación
776	0	8	_iPrinted edition: _z9781493908455
776	0	8	_iPrinted edition: _z9781493908431
776	0	8	_iPrinted edition: _z9781493946150
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0844-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI