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008			100301s2004 xxu| fo |||| 0|eng d
020			_a9781592598274
024	7		_a10.1385/1592598277 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aSB123.57 _b2004 EB
245	0	0	_aTransgenic Plants : _bMethods and Protocols _cedited by Leandro Peña
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XIV, 437 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v286
505	0		_aTransgenic Plants: An Historical Perspective -- Transformation -- Plant Transformation: Agrobacterium-Mediated Gene Transfer -- Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation -- Stable Transformation of Plant Cells by Particle Bombardment/Biolistics -- Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts -- Floral Dip: Agrobacterium-Mediated Germ Line Transformation -- Production of Transgenic Crops by the Floral-Dip Method -- Chloroplast Genetic Engineering to Improve Agronomic Traits -- Regeneration -- Organogenesis From Transformed Tomato Explants -- Genetic Transformation of Conifers Utilizing Somatic Embryogenesis -- Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues -- Genetic Transformation of Mature Citrus Plants -- Selection -- Selectable Markers: Antibiotic and Herbicide Resistance -- Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection -- Green Fluorescent Protein Quantification in Whole Plants -- Positive Selection -- Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems -- Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination -- Transgene Integration, Expression, and Localization -- The Study of Transgene Copy Number and Organization -- Analysis of Gene Expression in Transgenic Plants -- Transgene Integration: Use of Matrix Attachment Regions -- Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes -- Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction -- Risk Assessment -- Agrobacterium Persistence in Plant Tissues After Transformation -- Transgene Dispersal Through Pollen -- Transgenic Crops -- Transgenic Crops: The Current and Next Generations -- Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela.
520			_aTransgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems, including Agrobacterium tumefaciens, Agrobacterium rhizogenes, particle bombardment, electroporation, floral-dip, and chloroplast transformation, as well as the regeneration of whole transgenic plants by organogenesis and somatic embryogenesis from different explant cells/tissues and from such diverse plant species as tomato, cassava, conifers, and citrus. Also described are the use of selectable and reporter markers, positive selection (for maize transformation), and the controlled excision and removal of marker genes from both nuclei and plastids once transgenic shoots have been efficiently generated. Additional techniques employ quantitative real-time PCR, reverse transcription PCR, fluorescence in situ hybridization, and thermal asymmetric interlaced PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Transgenic Plants: Methods and Protocols offers both novice and experienced researchers a cutting-edge laboratory manual covering all aspects of plant genetic transformation and transgenic crops.
988			_aSpringer_Protocols_2004
650		7	_2embne _9157780 _aPlantas transgénicas
650		7	_2embne _9138044 _aGenética
776	0	8	_iPrinted edition: _z9781617374616
776	0	8	_iPrinted edition: _z9781489943637
776	0	8	_iPrinted edition: _z9781588292636
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598277 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI