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008			100301s2007 xxu| fo |||| 0|eng d
020			_a9781597452557
024	7		_a10.1007/978-1-59745-255-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP519.9 .M3 _b2007 EB
245	0	0	_aQuantitative Proteomics by Mass Spectrometry _cedited by Salvatore Sechi
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (X, 218 páginas) _b52 ilustraciones, 4 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v359
505	0		_aAcrylamide-A Cysteine Alkylating Reagent for Quantitative Proteomics -- Using Stable Isotope Tagging and Mass Spectrometry to Characterize Protein Complexes and to Detect Changes in Their Composition -- Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics -- Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope Labeling -- The Absolute Quantification Strategy -- Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling -- The Use of a Quantitative Cysteinyl-Peptide Enrichment Technology for High-Throughput Quantitative Proteomics -- An Isotope Coding Strategy for Proteomics Involving Both Amine and Carboxyl Group Labeling -- Proteolytic Labeling With 18O for Comparative Proteomics Studies -- Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening -- Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags -- Computational Analysis of Quantitative Proteomics Data Using Stable Isotope Labeling -- Quantitative Proteomic Analysis of Mammalian Organisms Using Metabolically Labeled Tissues -- Quantitative Proteomic Analysis of Phosphotyrosine-Mediated Cellular Signaling Networks.
520			_aQuantitative Proteomics by Mass Spectrometry, from the Methods in Molecular Biology™ series, is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers. Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS.
988			_aSpringer_Protocols_2007
650		7	_2embne _9141447 _aEspectrometría de masas
776	0	8	_iPrinted edition: _z9781617376481
776	0	8	_iPrinted edition: _z9781588295712
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-255-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI