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008			100301s1990 xxu| fo |||| 0|eng d
020			_a9781592596218
024	7		_a10.1385/0896031403 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP356.2 _b1990 EB
245	0	0	_aMolecular Neurobiological Techniques _cedited by Alan A. Boulton, Glen B. Baker, Anthony T. Campagnoni
250			_a1st edition 1990
264		1	_aTotowa, NJ _bHumana Press _c1990
300			_a1 recurso en línea (XVIII, 295 páginas) _b16 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v16
505	0		_aAnalysis of Brain mRNAs by Translation in Vitro -- Preparation of cDNA Libraries and Isolation and Analysis of Specific Clones -- Preparation and Use of Subtractive cDNA Hybridization Probes for cDNA Cloning -- Analysis of Brain-Specific Gene Products -- Isolation and Structure Determination of Genes -- Gene-Mapping Techniques -- Methods for Genetic Linkage Analyses -- Lineage Analysis and Immortalization of Neural Cells via Retrovirus Vectors -- Transgenic Mice in Neurobiological Research -- In Situ Hybridization.
520			_aIt goes without saying that the principles and techniques of molecular biology are having and will continue to have a major impact on investigations into nervous system structure and func tion. It is becoming increasingly apparent to neuroscientists in all subdisciplines that a working knowledge of the language, approaches, and techniques of molecular biology is indispensable for their work. For these reasons, the editors have decided to devote this volume of Neuromethods to the techniques of molecular biology and their application to neural systems. There currently exist a number of excellent reference technical manuals that de scribe molecular neurobiological techniques in great detail, and many of these are cited within the chapters included in this volume. It was not the intention of the editors or authors of this volume to duplicate these efforts. Rather, our intention was to present to the neuroscientist who is relatively unfamiliar with these methodologies an understanding of how specific techniques are used to approach major molecular neurobiological problems as well as a set of techniques that work in the laboratories of the individuals writing the chapters. In some cases, there are duplica tions of techniques these have been retained to illustrate the range of variability of the technique and/or the flexibility of the method to study different types of problems. We hope that the chapters will provide the reader with an understanding of the methods and their applicability to neurobiological problems; and, perhaps, suggest new directions for the reader's research efforts. Anthony T.
988			_aSpringer_Protocols_1990
650		7	_2embne _9139039 _aNeurobiología
776	0	8	_iPrinted edition: _z9781489940872
776	0	8	_iPrinted edition: _z9780896031401
776	0	8	_iPrinted edition: _z9781489940865
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896031403 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI