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020			_a9781592590483
024	7		_a10.1385/1592590489 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .M8 _b2000 EB
245	0	0	_aGlycoprotein : _bMethods and Protocols : The Mucins _cedited by Anthony P. Corfield
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XVII, 506 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v125
505	0		_aPurification of Mucin -- Isolation of Large Gel-Forming Mucins -- Preparation of Membrane Mucin -- Detection and Ouantitation of Mucin -- Histologically Based Methods for Detection of Mucin -- Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods -- The Gastrointestinal Adherent Mucous Gel Barrier -- Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling -- Separation, Identification, and Physical Characterization of Mucins -- Separation and Identification of Mucins and Their Glycoforms -- Heterogeneity and Size Distribution of Gel-Forming Mucins -- Rheology of Mucin -- Rheology of Mucin -- Mucin Peptide Analysis -- Amino Acid Analysis of Mucins -- Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation -- Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies -- Mucin Domains to Explore Disulfide-Dependent Dimer Formation -- Carbohydrate Structural Analysis of Mucins -- Monosaccharide Composition of Mucins -- O-Linked Oligosaccharide Chain Release and Fractionation -- Structural Analysis of Mucin-Type O-Linked Oligosaccharides -- Measurement of Sulfate in Mucins -- Mucin Biosynthesis -- Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study -- Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin -- Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis -- Mucin Precursors Identification and Analysis of Their Intracellular Processing -- Inhibition of Mucin Glycosylation -- O-Linked Chain Glycosyltransferases -- Mucin Gene Detection -- Mucin cDNA Cloning -- Northern Blot Analysis of Large mRNAs -- Southern Blot Analysis of Large DNA Fragments -- In Situ Hybridization Techniques for Localizing Mucin mRNA -- Detection of Mucin Gene Polymorphism -- Preparation of Antimucin Antibodies -- Polyclonal and Monoclonal Techniques -- Monoclonal Antibodies to Mucin VNTR Peptides -- Enzymatic Degradation of Mucins -- Mucinase Activity -- Proteinase Activity -- Glycosidase Activity -- Assays for Bacterial Mucin-Desulfating Sulfatases -- Mucin-Bacterial Interactions -- Mucin-Bacterial Binding Assays -- Growth of Mucin Degrading Bacteria in Biofilms -- Cellular And Humoral Responses To Mucins -- Generation of MUC1 CytotoxicT-Cells in Mice and Epitope Mapping -- Analysis of the Frequency of MHC-Unrestricted MUC1 -Specific CytotoxicT-Cells in Peripheral Blood by Limiting Dilution Assay -- Expression of MUC1 in Insect Cells Using Recombinant Baculovirus -- Mucin Antigen Presentation Using Dendritic Cells -- Detection of Humoral Immune Responses to Mucins.
520			_aAnthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. The techniques take advantage of the latest improvements in sensitivity and specificity of detection, and in the preparation of new reagents for specific biochemical detection. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for studying mucins. Its powerful techniques constitute a launching pad for those entering the field for the first time and a state-of-the-art collection for those already active in mucin research.
988			_aSpringer_Protocols_2000
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781617371493
776	0	8	_iPrinted edition: _z9780896037205
776	0	8	_iPrinted edition: _z9781489941398
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590489 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI