000 04175nam a22003855i 4500
999 _c392544
_d392544
001 392544
003 ES-MaUEC
005 20231202113149.0
006 a||||fo|||| 00| 0
007 cr nn 008mamaa
008 100715s2005 xxu| fo |||| 0|eng d
020 _a9781592599165
024 7 _a10.1385/1592599168
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRC280 .B6
_b2005 EB
245 0 0 _aMultiple Myeloma :
_bMethods and Protocols
_cedited by Ross D. Brown, P. Joy Ho
250 _a1st edition 2005
264 1 _aTotowa, NJ
_bHumana Press
_c2005
300 _a1 recurso en línea (XIV, 310 páginas)
_b70 ilustraciones, 1 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v113
505 0 _aMultiple Myeloma -- Immunophenotyping of Plasma Cells in Multiple Myeloma -- Plasma Cell Labeling Index -- Conventional Cytogenetics in Myeloma -- Multicolor Spectral Karyotyping in Multiple Myeloma -- Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization -- Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma -- Southern Blotting of IgH Rearrangements in B-Cell Disorders -- Identification and Assembly of V Genes as Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma -- Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma -- Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma -- Incomplete DJH Rearrangements -- Identification of Malignant Plasma Cells by mRNA In Situ Hybridization -- The SCID-hu Myeloma Model -- The 5T2MM Murine Model of Multiple Myeloma -- Determination of Telomerase Activity and Telomere Length -- Dendritic Cell/Myeloma Hybrid Vaccine -- Genetically Engineered Myeloma Cell Vaccine -- An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors -- Clonality Detection of Expanded T-Cell Populations in Patients With Multiple Myeloma -- Detecting Mismatch Repair Defects in Myeloma -- Methylation-Specific Polymerase Chain Reaction -- Making Sense of DNA Microarray Data.
520 _aAlthough there is no therapy that could be considered curative for patients with multiple myeloma, recent research has gained considerable momentum and yielded a real prospect for achieving a significant improvement in response to therapy. In Multiple Myeloma: Methods and Protocols, established researchers with hands-on experience detail a comprehensive set of classic and innovative methodologies used in many laboratories for the investigation of this disease. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology, which is used routinely in many laboratories around the world to determine prognosis, to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Multiple Myeloma: Methods and Protocols offers both novice and experienced researchers alike a gold-standard collection of proven techniques for investigating the pathogenesis of multiple myeloma and identifying new therapeutic targets.
988 _aSpringer_Protocols_2005
650 7 _2embne
_9140122
_aHematología
776 0 8 _iPrinted edition:
_z9781617375378
776 0 8 _iPrinted edition:
_z9781588293923
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599168
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_ean
_zSI