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008			140218s2014 xxu| fo |||| 0|eng d
020			_a9781627039802
024	7		_a10.1007/978-1-62703-980-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH450 _b2014 EB
245	0	0	_aSpliceosomal Pre-mRNA Splicing : _bMethods and Protocols _cedited by Klemens J. Hertel
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 427 páginas) _b66 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1126
505	0		_aThe Pre-mRNA Splicing Reaction -- Diversity and Evolution of Spliceosomal Systems -- Mechanisms of Spliceosomal Assembly -- Alternative Pre-mRNA Splicing -- Regulation of Alternative Pre-mRNA Splicing -- Introduction to Co-Transcriptional RNA Splicing -- Chromatin and Splicing -- Preparation of Splicing Competent Nuclear Extracts -- Preparation of Yeast Whole Cell Splicing Extract -- Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase -- In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract -- Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract -- In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation -- Isolation and Accumulation of Spliceosomal Assembly Intermediates -- Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function -- Expression and Purification of Splicing Proteins from Mammalian Cells -- Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis -- Cell-Based Splicing of Minigenes -- Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA -- Antisense Methods to Modulate Pre-mRNA Splicing -- Using Yeast Genetics to Study Splicing Mechanisms -- Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR -- Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing -- Computational Approaches to Mine Publicly Available Databases -- Approaches to Link RNA Secondary Structures with Splicing Regulation -- Methods to Study Splicing from High-Throughput RNA Sequencing Data -- Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq -- Predicting Alternative Splicing.
520			_aProviding a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo, manipulation of splicing events, and high-throughput and bioinformatic analyses of alternative splicing.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.
988			_aSpringer_Protocols_2014
650		7	_2embne _9141520 _aIngeniería genética
776	0	8	_iPrinted edition: _z9781627039796
776	0	8	_iPrinted edition: _z9781627039819
776	0	8	_iPrinted edition: _z9781493960446
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-980-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI