000			04310nam a22003975i 4500
999			_c392542 _d392542
001			392542
003			ES-MaUEC
005			20231202112735.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			170314s2017 xxu| fo |||| 0|eng d
020			_a9781493969401
024	7		_a10.1007/978-1-4939-6940-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2017 EB
245	0	0	_aSynthetic Protein Switches : _bMethods and Protocols _cedited by Viktor Stein
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 341 páginas) _b59 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1596
505	0		_aSynthetic Protein Switches: Theoretical and Experimental Considerations -- Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange -- Construction of Protein Switches by Domain Insertion and Directed Evolution -- Catalytic Amyloid Fibrils that Bind Copper to Activate Oxygen -- Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors -- Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modelling and Assessment -- Rational Design and Applications of Semi-Synthetic Modular Biosensors: SNIFITs and LUCIDs -- Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases -- Quantitative and Dynamic Imaging of ATM Kinase Activity -- Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains -- Protein and Protease Sensing by Allosteric Derepression -- DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor Complex Formation -- Engineering and Characterizing Synthetic Protease Sensors and Switches -- Characterizing Dynamic Protein-Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV -- Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light -- Light-Regulated Protein Kinases Based on the CRY2-CIB1 System -- Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels -- Primer-Aided Truncation for the Creation of Hybrid Proteins -- Engineering Small Molecule Responsive Split-Protein Kinases -- Directed Evolution Methods to Rewire Signaling Networks.
520			_aThis detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, β-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.
988			_aSpringer_Protocols_2017
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781493969388
776	0	8	_iPrinted edition: _z9781493969395
776	0	8	_iPrinted edition: _z9781493983452
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6940-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI