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008			130830s2013 xxu| fo |||| 0|eng d
020			_a9781627036139
024	7		_a10.1007/978-1-62703-613-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aSB317 .L43 _b2013 EB
245	0	0	_aLegume Genomics : _bMethods and Protocols _cedited by Ray J. Rose
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 316 páginas) _b53 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1069
505	0		_aThe Model Legume Genomes -- Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization -- Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes -- RNA-Seq for Transcriptome Analysis in Non-Model Plants -- Functional Analysis of Legume Genome Arrays -- Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing -- Determining Abundance of MicroRNAs and Other Small RNAs in Legumes -- Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines -- Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection -- High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants -- Isolation and Forward Genetic Analysis of Developmental Genes in Pea -- Gene Silencing in Medicago truncatula Roots Using RNAi -- Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius) -- Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens -- Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration -- Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins -- Proteomics and the Analysis of Nodulation -- Phosphoproteomic Analysis of Peptides -- Plant Metabolomics: From Experimental Design to Knowledge Extraction.
520			_aFeaturing current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
988			_aSpringer_Protocols_2013
650		7	_2embne _9143233 _aLegumbres _vGenetica
776	0	8	_iPrinted edition: _z9781627036146
776	0	8	_iPrinted edition: _z9781627036122
776	0	8	_iPrinted edition: _z9781493960248
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-613-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI