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020 _a9781493964338
024 7 _a10.1007/978-1-4939-6433-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP623
_b2016 EB
245 0 0 _aRNA Structure Determination :
_bMethods and Protocols
_cedited by Douglas H. Turner, David H. Mathews
250 _a1st edition 2016
264 1 _aNew York, NY
_bSpringer International Publishing
_c2016
300 _a1 recurso en línea (XV, 283 páginas)
_b81 ilustraciones, 66 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1490
505 0 _aCrumple: An Efficient Tool to Explore thoroughly the RNA Folding Landscape -- Secondary Structure Prediction of Single Sequences using RNAstructure -- Prediction of Secondary Structures Conserved in Multiple RNA Sequences -- Predicting RNA-RNA Interactions Using RNAstructure -- A Method to Predict the Structure and Stability of RNA/RNA Complexes -- STarMir Tools for Prediction of microRNA Binding Sites -- Traditional Chemical Mapping of RNA Structure in vitro and in vivo -- High-throughput Nuclease Probing of RNA Structures using FragSeq -- Mapping RNA Structure in vitro with SHAPE Chemistry and Next Generation Sequencing (SHAPE-Seq) -- Experiment-assisted Secondary Structure Prediction with RNAstructure -- RNA Secondary Structure Determination by NMR -- Modeling Small Non-canonical RNA Motifs with the Rosetta FARFAR Server -- Automated RNA 3D Structure Prediction with RNAComposer -- RNA 3D Structure Modeling by Combination of Template-Based Method ModeRNA, Template-Free Folding with SimRNA, and Refinement with QRNAS -- Exploring Alternative RNA Structure Sets using MC-FLashfold and db2cm -- NMR Methods for Characterization of RNA Secondary Structure -- The Quick and the Dead: A Guide to Fast Phasing of Small Ribozyme and Riboswitch Crystal Structures. .
520 _aThis volume provides protocols and procedures for determining and modeling RNA structure. Chapters guide the reader through protocols for RNA secondary structure prediction, single sequence modeling, Crumple, RNAstructure to model conserved secondary structures with multiple homologs, the prediction of bimolecular secondary structures with RNAstructure, STarMir, protocols for structure mapping, mapping data to constrain or restrain RNA secondary structure prediction with RNAstructure, unassigned NMR resonances, modeling protocols for Rosetta FARFAR, RNAComposer , ModeRNA, and MC-Fold. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. < Authoritative and Practical, RNA Structure Determination: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988 _aSpringer_Protocols_2016
650 7 _2embne
_9144940
_aARN
_vEstructura
776 0 8 _iPrinted edition:
_z9781493964314
776 0 8 _iPrinted edition:
_z9781493964321
776 0 8 _iPrinted edition:
_z9781493981984
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6433-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_ean
_zSI