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020 _a9781592598465
024 7 _a10.1385/1592598463
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aTP248.65 .E59
_b2005 EB
245 0 0 _aMicrobial Enzymes and Biotransformations
_cedited by Jose Luis Barredo
250 _a1st edition 2005
264 1 _aTotowa, NJ
_bHumana Press
_c2005
300 _a1 recurso en línea (XII, 320 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Biotechnology
_x1940-607X
_v17
505 0 _aMicrobial Cells and Enzymes A Century of Progress -- Enzyme Biosensors -- Directed Evolution of Enzymes -- L-Glutaminase as a Therapeutic Enzyme of Microbial Origin -- Enzymatic Production of D-Amino Acids -- Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides -- Food-Grade Corynebacteria for Enzyme Production -- Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase -- Enzymes in Modern Detergents -- Microbial Proteases -- Proteases Produced by Halophilic Bacteria and Archaea -- Microbial Pectinases -- Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes -- Glucose Dehydrogenase for the Regeneration of NADPH and NADH -- Acetate Kinase From Methanosarcina thermophila, a Key Enzyme for Methanogenesis -- Immobilization of Enzymes by Covalent Attachment -- Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads -- Methods for Bioencapsulation of Enzymes and Cells.
520 _aThe application of recombinant DNA technology to industrial fermentation and the production of enzymes over the last 20 years has produced a host of useful chemical and biochemical substances. In Microbial Enzymes and Biotransformations, leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Two overview chapters consider microorganisms as a source of metabolic and enzymatic diversity, and enzymes in the fast-moving field of enzyme biosensors. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Processes and Products, provides laboratory procedures for a wide range of processes and products mediated by microorganisms. Comprehensive and highly practical, Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.
988 _aSpringer_Protocols_2005
650 7 _2embne
_9138640
_aEnzimas
650 7 _2embne
_9138684
_aMicrobiología
650 7 _2embne
_9140806
_aADN
776 0 8 _iPrinted edition:
_z9781617374548
776 0 8 _iPrinted edition:
_z9781588292537
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598463
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b11/2023
_dz
_ean
_zSI