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008			121026s2012 xxu| fo |||| 0|eng d
020			_a9781627031134
024	7		_a10.1007/978-1-62703-113-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623 _b2012 EB
245	0	0	_aRecombinant and In Vitro RNA Synthesis : _bMethods and Protocols _cedited by Graeme L. Conn
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XIV, 274 páginas) _b56 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v941
505	0		_aPurification of RNA Expressed in vivo Inserted in a tRNA Scaffold.-Selective RNase H Cleavage of Target RNAs  from a tRNA Scaffold -- Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR -- General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE -- Preparation of Short RNA by in vitro Transcription -- Native RNA Purification by Gel Filtration Chromatography -- Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends -- Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends -- Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods.-Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA -- Affinity Purification of RNA using an ARiBo Tag -- Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing Experiments -- In Vitro Transcription of Modified RNAs -- End-Labeling Oligonucleotides with Chemical Tags After Synthesis -- High-Purity Enzymatic Synthesis of  Site-Specifically Modified tRNA -- Se-derivatized RNAs for X-ray Crystallography -- Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs -- Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC -- Splint Ligation of RNA with T4 DNA Ligase.
520			_aThe discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.
988			_aSpringer_Protocols_2012
650		7	_2embne _9140562 _aGenética humana
650		7	_2embne _9144940 _aARN
776	0	8	_iPrinted edition: _z9781627031127
776	0	8	_iPrinted edition: _z9781627031141
776	0	8	_iPrinted edition: _z9781493958306
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-113-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI