000			04051nam a22004095i 4500
999			_c392520 _d392520
001			392520
003			ES-MaUEC
005			20231118190403.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			131112s2014 xxu| fo |||| 0|eng d
020			_a9781627036887
024	7		_a10.1007/978-1-62703-688-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK881 _b2014 EB
245	0	0	_aPlant Metabolic Flux Analysis : _bMethods and Protocols _cedited by Martine Dieuaide-Noubhani, Ana Paula Alonso
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 365 páginas) _b75 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1090
505	0		_aApplication of Metabolic Flux Analysis to Plants -- Metabolic Network Reconstruction and Their Topological Analysis -- 14C pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism -- Optimization of Steady-state 13C-labeling Experiments for Metabolic Flux Analysis -- Quantification of 13C Enrichments and Isotopomer Abundances for Metabolic Flux analysis using 1D NMR Spectroscopy -- Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR -- Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS -- Quantifying 13C-labeling in Free Sugars and Starch by GC-MS -- Liquid Chromatography Tandem Mass Spectrometry for Measuring 13C-labeling in Intermediates of the Glycolysis and Pentose-phosphate Pathway -- In Vivo NMR for 13C metabolic  Flux Analysis -- Steady State and Instationary Modeling of Proteinogenic and Free Amino Acid Isotopomers for Flux Quantification -- Isotopically Nonstationary MFA (INST-MFA) of Autotrophic Metabolism -- Simulating Labeling to Estimate Kinetic Parameters for Flux Control Analysis -- High-throughput Data Pipelines for Metabolic Flux Analysis in Plants -- Analysis of Enzyme Activities -- Analytical Kinetic Modeling: A Practical Procedure -- Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling -- Flux Variability Analysis: Application to Developing Oilseed Rape Embryos using Toolboxes for Constraint-Based Modeling -- Plant Genome-Scale Modeling and Implementation -- 34S and 15N Labelling to Model S and N Flux in Plants and Determine the Different Components of N and S use Efficiency -- Ecophysiological Process-based Model to Simulate Carbon Fluxes in Plants.
520			_aIn Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.       Authoritative and practical Plant Metabolic Flux Analysis,  seeks to aid scientists in the further study of cutting-edge protocols and methodologies that are crucial to getting ahead in MFA.
988			_aSpringer_Protocols_2014
650		7	_2embne _9168920 _aPlantas _xMetabolismo
650		7	_2embne _9138639 _aBioquímica
776	0	8	_iPrinted edition: _z9781627036894
776	0	8	_iPrinted edition: _z9781627036870
776	0	8	_iPrinted edition: _z9781493960507
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-688-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI