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008			170816s2017 xxu| fo |||| 0|eng d
020			_a9781493971695
024	7		_a10.1007/978-1-4939-7169-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH443 _b2017 EB
245	0	0	_aSite-Specific Recombinases : _bMethods and Protocols _cedited by Nikolai Eroshenko
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 342 páginas) _b50 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1642
505	0		_aGenerating Genetically Modified Mice: A Decision Guide -- Direct Generation of Conditional Alleles using CRISPR/Cas9 in Mouse Zygotes -- Building Cre Knockin Rat Lines using CRISPR/Cas9 -- Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases -- Use of the DICE Dual Integrase Cassette Exchange System -- Ligand-controlled Site-specific Recombination in Zebrafish -- Injection-based Delivery of Cell Permeable Peptide-tagged Cre -- Viral Delivery of GFP-dependent Recombinases to the Mouse Brain -- Recombinase-mediated Cassette Exchange using Adenoviral Vectors -- Marker Removal in Transgenic Plants using Cre recombinase Delivered with Potato Virus X -- Nanoparticle-mediated Recombinase Delivery into Maize -- Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines -- FLPing Genes On and Off in Drosophila -- Imaging Neural Architecture in Brainbow Samples -- Purification and in vitro characterization.
520			_aThis books aims to provide an up-to-date guide to using recombinases in the laboratory.  Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.
988			_aSpringer_Protocols_2017
650		7	_2embne _9140562 _aGenética humana _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493971671
776	0	8	_iPrinted edition: _z9781493971688
776	0	8	_iPrinted edition: _z9781493984053
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7169-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI