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020			_a9781592597659
024	7		_a10.1385/1592597653 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR100 _b2004 EB
245	0	0	_aEnvironmental Microbiology : _bMethods and Protocols _cedited by John F. T. Spencer, Alicia L. Ragout de Spencer
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XIV, 426 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Biotechnology _x1940-607X _v16
505	0		_aCommunities and Biofilms -- Isolation and Molecular Characterization of Seawater Bacteria -- Reverse Sample Genome Probing to Monitor Microbial Communities -- T-RFLP Analysis -- Assessing Bacterial DNA Content in Aquatic Systems -- Multiplexed Identification and Quantification of Analyte DNAs in Environmental Samples Using Microspheres and Flow Cytometry -- Molecular Characterization of Microbial Communities From Marine Environments -- Yeasts -- Aeromonads in Environmental Waters -- Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface Ground Water -- Bench Scale Flow Cell for Nondestructive Imaging of Biofilms -- Fermented Milks -- ?-Galactosidase Assay in Fermented Soymilk Products -- Considerations to Avoid the Overestimation of Exopolysaccharides Produced by Lactic Acid Bacteria -- Determination of Oligosaccharides in Fermented Soymilk Products by High-Performance Liquid Chromatography -- Evaluation of Minimal Nutritional Requirements of Lactic Acid Bacteria Used in Functional Foods -- Nuclei Acids, Recovery, and Determination -- A Simple Method for Obtaining DNA Suitable for RAPD Analysis From Azospirillum -- PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmental Bacteria Isolates -- A DNA-DNA Hybridization Method for the Detection and Quantification of Specific Bacterial Taxa in Natural Environments -- 13C and 1H NMR Study of the Glycogen Futile Cycle in Fibrobacter succinogenes -- Chemical Analysis and Biological Removal of Wood Lipids Forming Pitch Deposits in Paper Pulp Manufacturing -- Propolis -- Enzymatic Saccharification of Cellulosic Materials -- Molecular Identification of Microbial Populations in Petroleum-Contaminated Groundwater -- Identification of Copper-Resistant Microorganisms by PCR -- Selection of Tolerant Heavy Metal Yeasts From Different Polluted Sites -- Medium for Differential Enumeration of Lactobacillus casei and Lactobacillus acidophilus From Lyophilized Mixed Cultures -- Silk as a Means of Recovering Bacteriocin From a Culture Medium -- Optimization of a Method for Isolating Plasmid DNA From Lactic Acid Bacteria From Wine -- Simultaneous and Sequential Methods to Study Interactions Between Yeast and Lactic Acid Bacteria From Wine -- Method for Determining Lindane Concentration in Water and Solid Samples -- Extracellular Hydrolytic Enzymes Produced by Yeasts -- Extracellular Hydrolytic Enzymes Produced by Phytopathogenic Fungi -- An Analysis of Microorganisms in Environments Using Denaturing Gradient Gel Electrophoresis -- Testing for Evolutionary Correlations in Microbiology Using Phylogenetic Generalized Least Squares -- Conjugated Linoleic Acid Detection Produced by Dairy Starter Cultures -- Reviews -- Techniques for Manipulating the Bacterial Endophyte Bacillus mojavensis -- Engineering of Bacteria Using the Vitreoscilla Hemoglobin Gene to Enhance Bioremediation of Aromatic Compounds -- The Use of Chemical Shift Reagents and 23Na NMR to Study Sodium Gradients in Microorganisms -- Denaturing Gradient Gel Electrophoresis (DGGE) as a Fingerprinting Tool for Analyzing Microbial Communities in Contaminated Environments.
520			_aUnderstanding the relationship between a microorganism and its environment is essential to the successful manipulation of industrial, biochemical, and medical processes. In Environmental Microbiology: Methods and Protocols, highly practiced experimentalists who often have perfected the methods they write about describe readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity found in remote environments. The protocols for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms, are provided for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals. Review articles discuss the endophytic bacterium Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, and the use of chemical shift reagents and Na-NMR to study sodium gradients in microorganisms. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Environmental Microbiology: Methods and Protocols offers microbiological researchers a powerful set of techniques for investigating and understanding microorganisms in their native environments.
988			_aSpringer_Protocols_2004
650		7	_2embne _9150106 _aEcología microbiana
650		7	_2embne _9138684 _aMicrobiología
776	0	8	_iPrinted edition: _z9781617373701
776	0	8	_iPrinted edition: _z9781588291165
776	0	8	_iPrinted edition: _z9781489939258
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597653 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI