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007			cr nn 008mamaa
008			110414s2009 xxu| fo |||| 0|eng d
020			_a9781597454131
024	7		_a10.1007/978-1-59745-413-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP88.23 _b2009 EB
245	0	0	_aExtracellular Matrix Protocols : _bSecond Edition _cedited by Sharona Even-Ram, Vira Artym
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 324 páginas) _b59 ilustraciones, 9 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v522
505	0		_aMolecular Biology -- Retroviral delivery of ECM genes -- Tissue-Specific KO of ECM Proteins -- Recombinant Collagen Trimers from Insect Cells and Yeast -- Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag -- Preparation of recombinant fibronectin fragments for functional and structural studies -- Quantitative Determination of Collagen Cross-links -- Biochemical and biophysical analysis -- ECM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy -- Atomic Force Microscopy Measurements of Intermolecular Binding Forces -- Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy -- Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS) -- ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy -- Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates -- Solid Phase Assays for Studying ECM Protein-Protein Interactions -- Cell Adhesion Assays -- Cell biology assays -- ECM Degradation Assays for Analyzing Local Cell Invasion -- Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration -- Fibrin Gel Model for Assessment of Cellular Contractility -- Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis) -- Stromagenesis During Tumorigenesis: Characterization of Tumor-associated Fibroblasts and Stroma-derived 3D Matrices -- Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes -- Organ models -- ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development -- Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice -- Tissue Engineering -- Characterizing ECM Production by Cells Encapsulated in Hydrogels -- Tissue Engineering and Cell-Populated Collagen Matrices.
520			_aThe study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Protocols: Second Edition, expert researchers explore this developing field and provide a number of techniques which can be reproduced in most cell biology labs. This edition contains methods that represent cutting edge unique technologies which are well worth exposure to the broad field and may encourage collaboration with expert labs. Chapters include information on molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, and cell biology methods for assessment of cell behavior, matrix assembly and tissue engineering applications. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Extracellular Matrix Protocols: Second Edition investigates the diverse roles of the extracellular matrix, providing important tools for all researchers in the ECM community.
988			_aSpringer_Protocols_2009
650		7	_2embne _9139622 _aCitogenética
650		7	_2embne _9139103 _aBiología molecular _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607612681
776	0	8	_iPrinted edition: _z9781617378676
776	0	8	_iPrinted edition: _z9781588299840
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-413-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI