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008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592592340
024	7		_a10.1385/1592592341 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP624.5.D726
245	1	0	_aDNA Arrays : _bMethods and Protocols _cedited by Jang B. Rampal
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XI, 264 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v170
505	0		_aDNA Microarrays -- Gel-Immobilized Microarrays of Nucleic Acids and Proteins -- Sequencing by Hybridization Arrays -- Ethical Ramifications of Genetic Analysis Using DNA Arrays -- Photolithographic Synthesis of High-Density Oligonucleotide Arrays -- Automated Genotyping Using the DNA MassArray™ Technology -- Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules -- Printing DNA Microarrays Using the Biomek® 2000 Laboratory Automation Workstation -- Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays -- Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays -- DNA Sequencing by Hybridization with Arrays of Samples or Probes -- Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents -- Low-Resolution Typing of HLA-DQA1 Using DNA Microarray -- Gene Expression Analysis on Medium-Density Oligonucleotide Arrays -- Use of Bioinformatics in Arrays -- Confocal Scanning of Genetic Microarrays -- Business Aspects of Biochip Technologies.
520			_aMicroarray technology, which permits the rapid, simultaneous, and highly sensitive analysis of large numbers of biological samples, is now coming into widespread use for advanced research on gene expression, mutation analysis, proteomics, and gene sequencing. In DNA Arrays: Methods and Protocols, Jang Rampal and a authoritative panel of researchers, engineers, and technologists explain in detail how to design and construct these DNA arrays, as well as how to hybridize them with biological samples for analysis. In step-by-step instructions these experts detail not only how to attach or print arrays on various matrices, but also biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies, and data collection and analysis (bioinformatics). Additional topics covered include genotyping, sequencing by hybridization, antisense reagents, HLA-DQA typing techniques, and gene expression analysis. Rounding out the technical presentation are three chapters that review the history of microarrays, the ethical ramifications of genetic analysis using DNA arrays, and the business aspects of biochip technologies. Forward-looking and state-of-the-art, DNA Arrays: Methods and Protocols provides all investigators engaged in biological and biomedical research the full range of effective, readily reproducible microarray techniques needed today to analyze on a large scale the many different genes and gene sequences now available from the Human Genome Project.
988			_aSpringer_Protocols_2001
650		7	_2embne _9670812 _aMicromatrices de ADN
776	0	8	_iPrinted edition: _z9781617372049
776	0	8	_iPrinted edition: _z9780896038226
776	0	8	_iPrinted edition: _z9781489944009
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592341 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eIG _zSI