000			04231nam a22003975i 4500
999			_c392501 _d392501
001			392501
003			ES-MaUEC
005			20231010090702.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s2003 xxu| o |||| 0|eng d
020			_a9781592593675
024	7		_a10.1385/1592593674 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR186.6.S94 _b2003 EB
245	0	0	_aSuperantigen Protocols _cedited by Teresa Krakauer
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XII, 259 páginas) _b59 ilustraciones, 6 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v214
505	0		_aSuperantigens -- Expression, Purification, and Detection of Novel Streptococcal Superantigens -- Flow Cytometric Detection of MMTV Superantigens -- Spectrophotometric Methods for the Determination of Superantigen Structure and Stability -- Binding Kinetics of Superantigen with TCR and MHC Class II -- Directed Evolution of T-Cell Receptors for Binding Superantigens -- Analysis of Superantigen Binding to Soluble T-Cell Receptors -- Role of Accessory Molecules in the Superantigen-Induced Activation of Peripheral Blood T Cells -- T-Lymphocyte Activation Induced by Staphylococcal Enterotoxin Superantigens -- Measurement of Proinflammatory Cytokines and T-Cell Proliferative Response in Superantigen-Activated Human Peripheral Blood Mononuclear Cells -- RNase Protection Assay for the Study of the Differential Effects of Therapeutic Agents in Suppressing Staphylococcal Enterotoxin B-Induced Cytokines in Human Peripheral Blood Mononuclear Cells -- Staining Protocol for Superantigen-Induced Cytokine Production Studied at the Single-Cell Level -- Assessment of Specific T-Cell Activation by Superantigens -- Superantigen-Induced Changes in Epithelial Ion Transport and Barrier Function -- Pyrogenic, Lethal, and Emetic Properties of Superantigens in Rabbits and Primates.
520			_aSuperantigens, a class of proteins produced by bacteria and microbes, cause diseases ranging from food poisoning to toxic shock, but can also be used to understand these pathologies and devise therapeutic agents to combat them. In Superantigen Protocols, leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of these potent activators of the immune system. The protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level. Additional techniques for the discovery of new activators of the immune system, the development of new therapeutics for the suppression of superantigen-induced effects, and the treatment of inflammatory diseases are detailed. Comprehensive and cutting-edge, Superantigen Protocols provides a broad range of novel immunological and biochemical techniques that can be successfully applied not only to the structural, functional, and clinical properties of superantigens, but also to the many varieties of such proteins and to new cellular systems.
988			_aSpringer_Protocols_2003
650		7	_2embne _9138531 _aAntígenos
776	0	8	_iPrinted edition: _z9781489938237
776	0	8	_iPrinted edition: _z9780896039841
776	0	8	_iPrinted edition: _z9781489938220
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593674 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eIG _zSI