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008			100301s2000 xxu| o |||| 0|eng d
020			_a9781592590537
024	7		_a10.1385/1592590535 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552.T72 _b2000 EB
245	o	0	_aTransforming Growth Factor-Beta Protocols _cedited by Philip H. Howe
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (X, 165 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v142
505	0		_aIn Vitro Assays for Measuring TGF? Growth Stimulation and Inhibition -- Measurements of Active TGF? Generated by Culture Cells -- Methods for Iodination of Active TGF?, TGF? Receptor Crosslinking and Immunoprecipitation of TGF?-Receptor Complexes -- Detection of TGF? in Body Fluids and Tissues -- Analysis of TGF?-Mediated Synthesis of Extracellular Matrix Components -- Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction -- Promoter Analysis of TGF?-Responsive Genes by Transient Transfection and Deletional/Mutational Analysis -- Regulation of AP-1 Activity by TGF? -- CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors -- Activation of the Mitogen-Activated Protein Kinase Pathway by TGF? -- 11 Detection of TGF? Type II Receptor Hotspot Mutations: The BAT-RII Assay -- Mutation Detection in the TGF? Receptors and SMAD Genes: RT-PCR and Sequencing -- Analysis of TGF?-Inducible Apoptosis.
520			_aThe recent isolation and identification of the TGFß receptors, as well as their intracellular mediating proteins, has driven the development of many new methodologies and techniques for the study of TGFß. In Transforming Growth Factor-Beta Protocols, Philip H. Howe and a group of well-versed experimentalists present the first major collection of indispensable classic and cutting-edge TGFß assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGFß to methodologies for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature. Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists-both expert and novice-with time-tested methods for the identification and analysis of the signal transduction pathways by which TGFß induces and modulates physiological behavior.
988			_aSpringer_Protocols_2000
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781489943989
776	0	8	_iPrinted edition: _z9780896036468
776	0	8	_iPrinted edition: _z9781489943972
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590535 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eIG _zSI