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020 _a9781607618065
024 7 _a10.1007/978-1-60761-806-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRS189
_b2010 EB
245 1 0 _aTissue Microarrays :
_bMethods and Protocols
_cedited by Ronald Simon
250 _a1st edition 2010
264 1 _aTotowa, NJ
_bHumana Press
_c2010
300 _a1 recurso en línea (X, 193 páginas)
_b47 ilustraciones, 4 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v664
505 0 _aApplications of Tissue Microarray Technology -- Quality Aspects of TMA Analysis -- Representativity of TMA Studies -- Recipient Block TMA Technique -- Protocol for Constructing Tissue Arrays by Cutting Edge Matrix Assembly -- Hypodermic Needle Without Recipient Paraffin Block Technique -- Resin Technologies: Construction and Staining of Resin TMA's -- Tissue Microarrays from Frozen Tissues-OCT Technique -- An Alternative Technology to Prepare Tissue Microarray Using Frozen Tissue Samples -- Building "Tissue" Microarrays from Suspension Cells -- Tissue Microarrays from Biopsy Specimens -- Immunohistochemical Analysis of Tissue Microarrays -- DNA Copy Number Analysis on Tissue Microarrays -- RNA Expression Analysis on Formalin-Fixed Paraffin-Embedded Tissues in TMA Format by RNA In Situ Hybridization -- Automated Analysis of Tissue Microarrays -- Digital Microscopy for Boosting Database Integration and Analysis in TMA Studies -- From Gene to Clinic: TMA-Based Clinical Validation of Molecular Markers in Prostate Cancer.
520 _aToday's tissue microarray (TMA) method presents as a modern high-tech technology, one which allows for the linking of clinical data to the tissues that are combined on one slide. In Tissue Microarrays: Methods and Protocols, expert researchers explore the current world of TMA making and TMA applications, providing insight into the inherent and complex aspects of the most popular assays used for in-situ tissue analysis. Chapters examine the range of TMA techniques that allow for a large number of tissues to be included in one TMA, preserve the integrity of donor tissue blocks, and present a highly organized array pattern that allows for the reliable allocation of clinical data to individual tissue spots. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and ground-breaking, Tissue Microarrays: Methods and Protocols serves as an essential handbook for pathologists, molecular biologists, researchers in the life sciences, as well as physicians, a reflection of the various applications of current TMA technology.
988 _aSpringer_Protocols_2010
650 7 _2embne
_9140254
_aTejidos
776 0 8 _iPrinted edition:
_z9781607618058
776 0 8 _iPrinted edition:
_z9781607618072
776 0 8 _iPrinted edition:
_z9781493961030
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-806-5
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_eIG
_zSI