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020 _a9781493917853
024 7 _a10.1007/978-1-4939-1785-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH588.S83
_b2015 EB
245 0 0 _aStem Cell Protocols
_cedited by Ivan N. Rich
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XI, 281 páginas)
_b56 ilustraciones, 47 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1235
505 0 _aA Short Primer in Stem Cell Biology -- Measurement of Hematopoietic Stem Cell Proliferation, Self-Renewal and Expansion Potential -- Measuring the Aging Process in Stem Cells -- Measuring the Potency of a Stem Cell Therapeutic -- Culturing Protocols for Human Multipotent Adult Stem Cells -- The Isolation of Murine Bone Marrow Scavenging Sinusoidal Endothelial Cells -- Analysis of Circadian Rhythms in Embryonic Stem Cells -- Measuring Stem Cell Circadian Rhythms -- Cryopreservation of Human Pluripotent Stem Cells: A General Protocol -- Biological Differences Between Native and Cultured Mesenchymal Stem Cells - Implications for Therapies -- The Use of Multiparameter Flow Cytometry and Cell Sorting to Characterize Native Human Bone Marrow Mesenchymal Stem Cells (MSC) -- High Yield Recovery of Equine MEsenchymal Stem Cells from Umbilical Cord Matrix/Wharton's Jelly Using a Semi-Automated Process -- Isolation and Functional Assessment of Cutaneous Stem Cells -- Isolation of Adult Stem Cell Populations from the Human Cornea -- Advanced Imaging and Tissue Engineering of the Human Limbal Epithelial Stem Cell Niche -- Isolation and Characterization of Stem Cells in the Adult Mammalian Ovary -- Clonal Culture of Adult Mouse Lung Epithelial Stem/Progenitor Cells -- Culture and Characterization of Mammary Cancer Stem Cells in Mammospeheres -- Isolation and Culture of Primary Glioblastoma Cells from Human Tumor Specimens.
520 _aThis volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.
988 _aSpringer_Protocols_2015
650 7 _2embne
_9160528
_aCélulas madre
776 0 8 _iPrinted edition:
_z9781493917860
776 0 8 _iPrinted edition:
_z9781493917846
776 0 8 _iPrinted edition:
_z9781493955312
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1785-3
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b09/2023
_dz
_eIG
_zSI