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008			150731s2016 xxu| o |||| 0|eng d
020			_a9781493929269
024	7		_a10.1007/978-1-4939-2926-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP609.C93 _b2016 EB
245	0	0	_aCyclin-Dependent Kinase (CDK) Inhibitors : _bMethods and Protocols _cedited by Mar Orzáez, Mónica Sancho Medina, Enrique Pérez-Payá
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (X, 227 páginas) _b42 ilustraciones, 22 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1336
505	0		_aImmunoprecipitation of CDK-Cyclin Complexes for Determination of Kinase Activity -- Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation -- Expression and Purification of Recombinant CDKs: CDK7, CDK8 and CDK9 -- Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination -- Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors --  Protein-Protein Interaction Inspire the De Novo Design of Cyclin-Dependent Kinases Peptide Inhibitors -- Identification of Cyclin A Binders with a Fluorescent Peptide Sensor -- Cell Synchronization Techniques to Study the Action of CDK Inhibitors -- Analysis of CDK Inhibitors Action on Mitochondria-Mediated Apoptosis -- Evaluating the Effects of CDK Inhibitors in Ischemia-Reperfusion Injury Models -- Assessing Cell Cycle Independent Function of the CDK Inhibitor p21CDKN1A in DNA Repair -- Drug Delivery Strategies of Chemical CDK Inhibitors -- Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs -- Evaluating Chemical CDK Inhibitors as Cell Death Inducers -- Models for the Study of the Crosstalk between Inflammation and Cell Cycle -- Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors.
520			_aThis volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.
988			_aSpringer_Protocols_2016
650		7	_2embne _9510854 _aProteína cinasas
650		7	_2embne _9162841 _aInmunidad celular
776	0	8	_iPrinted edition: _z9781493929252
776	0	8	_iPrinted edition: _z9781493929276
776	0	8	_iPrinted edition: _z9781493943685
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2926-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eIG _zSI