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020 _a9781597454308
024 7 _a10.1007/978-1-59745-430-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP552.P4
_b2007 EB
245 0 0 _aPeptide Characterization and Application Protocols
_cedited by Gregg B. Fields
250 _a1st edition 2007
264 1 _aTotowa, NJ
_bHumana Press
_c2007
300 _a1 recurso en línea (XII, 342 páginas)
_b102 ilustraciones, 4 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v386
505 0 _aCharacterization -- HPLC Analysis and Purification of Peptides -- Identification of Proteins Based on MS/MS Spectra and Location of Posttranslational Modifications -- Determination of Phosphorylated and O-Glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature -- Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein-Coupled Receptor -- Applications -- Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors -- Proteolytic Profiling of the Extracellular Matrix Degradome -- ?-Amyloid Protein Aggregation -- Radiometal-Labeled Somatostatin Analogs for Applications in Cancer Imaging and Therapy -- Cell-Penetrating Proline-Rich Peptidomimetics -- Peptide-Mediated Targeting of Liposomes to Tumor Cells -- Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells -- Broth Microdilution Antibacterial Assay of Peptides -- Depsipeptide Synthesis.
520 _aPeptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.
988 _aSpringer_Protocols_2007
650 7 _2embne
_9146243
_aPéptidos
776 0 8 _iPrinted edition:
_z9781617376375
776 0 8 _iPrinted edition:
_z9781588295507
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-430-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_eIG
_zSI