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008			101109s2011 xxu| o |||| 0|eng d
020			_a9781607619628
024	7		_a10.1007/978-1-60761-962-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC931.O67 _b2011 EB
245	0	0	_aEmbryonic Stem Cell Therapy for Osteo-Degenerative Diseases : _bMethods and Protocols _cedited by Nicole I. Nieden
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XII, 336 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v690
505	0		_aEmbryonic Stem Cells for Osteo-Degenerative Diseases -- Methods for Culturing Mouse and Human Embryonic Stem Cells -- Serum-Free and Feeder free Culture Conditions for Human Embryonic Stem Cells -- Functional Assays for Human Embryonic Stem Cell Pluripotency -- Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells -- Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters -- Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives -- Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers -- Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology -- Methods for Embryoid Body Formation: The Microwell Approach -- Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview -- Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells -- Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the "Raclure" Method -- Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes -- Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds -- Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells -- Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield -- Identification of Osteoclasts in Culture -- Analysis of Glycosaminoglycans in Stem Cell Glycomics -- Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent -- Measurement and Illustration of Immune Interaction after Stem Cell Transplantation.
520			_aEmbryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.
988			_aSpringer_Protocols_2011
650		7	_2embne _9138753 _aArtritis
650		7	_2embne _9138760 _aArtrosis
776	0	8	_iPrinted edition: _z9781607619611
776	0	8	_iPrinted edition: _z9781607619635
776	0	8	_iPrinted edition: _z9781493957231
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-962-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b10/2023 _dz _eIG _zSI