000			04144nam a22003975i 4500
999			_c392470 _d392470
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003			ES-MaUEC
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006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s1995 xxu| o |||| 0|eng d
020			_a9781592595310
024	7		_a10.1385/0896033023 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR82.R45 _b1995 EB
245	0	0	_aAgrobacterium Protocols _cedited by Kevan M. A. Gartland, Michael R. Davey
250			_a1st edition 1995
264		1	_aTotowa, NJ _bHumana Press _c1995
300			_a1 recurso en línea (XIV, 417 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v44
505	0		_aGrowth and Storage of Agrobacterium -- Simple Cultural Tests for Identification of Agrobacterium Biovars -- Agrobacterium Virulence -- Agrobacterium tumefaciens Chemotaxis Protocols -- Effect of Acetosyringone on Growth and Oncogenic Potential of Agrobacterium tumefaciens -- Binary Ti Plasmid Vectors -- Leaf Disk Transformation -- Agrobacterium-Mediated Transformation of Protoplasts from Oilseed Rape (Brassica napus L.) -- Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.) -- Peanut Transformation -- Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants -- Agrobacterium-Mediated Transformation of Potato Genotypes -- Agrobacterium-Mediated Transformation of Soft Fruit Rubus, Ribes, and Fragaria -- High-Frequency and Efficient Agrobacterium-Mediated Transformation ofArabidopsis thaliana Ecotypes "C24" and "Landsberg erecta" Using Agrobacterium tumefaciens -- Transformation Protocols for Broadleaved Trees -- Agrobacterium-Mediated Antibiotic Resistance for Selection of Somatic Hybrids -- Histochemical GUS Analysis -- Fluorometric GUS Analysis for Transformed Plant Material -- The Detection of Neomycin Phosphotransferase Activity in Plant Extracts -- The Plant Oncogenes rolA, B, and C from Agrobacterium rhizogenes -- Quantifying Polyamines in Agrobacterium rhizogenes Strains and in Ri Plasmid Transformed Cells -- IAA Analysis in Transgenic Plants -- Quantifying Phytohormones in Transformed Plants -- Manipulating Photosynthesis in Transgenic Plants -- Gene Activation by T-DNA Tagging -- Assessing Cadmium Partitioning in Transgenic Plants -- Overexpression of Chloroplastic Cu/Zn Superoxide Dismutase in Plants -- Agroinfection -- T-DNA Transfer to Maize Plants -- Use of Cosmid Libraries in Plant Transformations -- Regulation of Agrobacterium Gene Manipulation -- Transgenic Oilseed Rape -- Electroporation Protocols for Agrobacterium.
520			_aAgrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants. Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.
988			_aSpringer_Protocols_1995
650		7	_2embne _9144590 _aRhizobium
776	0	8	_iPrinted edition: _z9781489927996
776	0	8	_iPrinted edition: _z9781489927989
776	0	8	_iPrinted edition: _z9780896033023
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033023 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI